结直肠癌p53基因点突变和蛋白表达的研究

目的了解中国人结直肠癌ｐ５３基因的突变谱，探讨聚合酶链反应－单链构象多态（ＰＣＲ－ＳＳＣＰ）银染技术用于研究结直肠癌中ｐ５３基因突变的可行性。方法应用ＰＣＲ－ＳＳＣＰ银染技术检测４１例结直肠癌ｐ５３基因突变，以ＡＢＣ免疫组织化学染色检测结直肠癌Ｐ５３蛋白的表达。结果３４％（１４／４１）的病例显示有ｐ５３基因突变，其中外显子５，６，７和８各有４，１，５和４例突变。２０例有Ｐ５３蛋白异常表达，阳性率为４９％。结论导致Ｐ５３蛋白异常堆积的ｐ５３基因突变是结直肠癌的一种常见的分子结构改变，可能在结直肠癌的发生和发展中起着重要的作用。ＰＣＲ－ＳＳＣＰ银染技术是一简便、快速、有效的检测基因点突变的方法

Mutation of p53 gene and expression of p53 protein in human colorectal cancer

Objective To understand the mutation spectrum of p53 gene of colorectal cancer in Chinese and to determine if the PCR SSCP silver staining technique is practicable. Methods Detected p53 gene mutation by PCR SSCP silver staining technique and protein expression by ABC immunohistochemistry. Results p53 gene mutations were detected in 34%(14 of 41) of cases. The mutation figure of p53 gene was 4,1,5,4 in exon 5,6,7,8, respectively. Nuclear accumulation of P53 protein was observed in 20 out of 41 cases of colorectal cancer(49%). Conclusions p53 gene mutation, which results in abnormal protein accumulation, was a frequent molecular structural change and may play an important role during colorectal carcinogenesis. Detection of point mutation with PCR SSCP silver staining technique is convenient, rapid and reliable in the screening of point mutation of genes.