| RCS大鼠自然病程中视网膜边缘生发区细胞增殖及Shh/Ptc信号途径改变的观察 |
| |
| 引用本文： | 蹇骞,徐海伟,谢汉平,阴正勤.RCS大鼠自然病程中视网膜边缘生发区细胞增殖及Shh/Ptc信号途径改变的观察[J].第三军医大学学报,2009,31(11). |
| |
| 作者姓名： | 蹇骞 徐海伟 谢汉平 阴正勤 |
| |
| 作者单位： | 1. 第三军医大学西南医院全军眼科中心,重庆,400038
2. 第三军医大学基础医学部生理学教研室,重庆市神经科学研究所,重庆,400038 |
| |
| 基金项目： | 国家重点基础研究发展规划(973计划) |
| |
| 摘 要： | 目的 观察遗传性视网膜色素变性RCS大鼠病程发展中视网膜边缘生发区Shh/Ptc信号途径改变及其与该区细胞增殖能力变化的关系.方法 RCS大鼠按出生后病程变化分为15、30、60、90 d 4组(免疫组化每组4只,PCR每组3只),以同龄含色素的正常大鼠(Long-Evan's 大鼠)作为正常对照.视网膜组织切片DAPI荧光染色观察外核层(outer nuclear layer,ONL)厚度的变化;视网膜细胞增殖标记Ki67免疫荧光染色观察视网膜边缘生发区细胞增殖能力的变化;荧光定量PCR检测视网膜边缘生发区Shh/Ptc信号途径中关键分子Shh、Ptc1、Smo和Gli1 mRNA表达水平.结果 ①RCS大鼠从第15天开始,随着年龄的增加视网膜ONL逐渐变薄,第90天ONL几乎消失.②正常对照组大鼠视网膜边缘生发区Ki67阳性细胞较少,与同龄正常对照组大鼠相比,出生30、60 d组大鼠Ki67阳性细胞数均显著增多(P＜0.05);大鼠各年龄组间比较,60 d组Ki67阳性细胞数明显增多(P＜0.01).③60 d组大鼠视网膜边缘生发区Shh、Ptc1、Smo和Gli1mRNA表达水平明显上调.结论 出生后60 d RCS大鼠视网膜边缘生发区Shh/Ptc信号途径的激活,可能刺激了该部位细胞短期的增殖.
|
| 关 键 词： | 视网膜色素变性 视网膜干细胞 细胞增殖 Shh/Ptc信号途径 荧光定量PCR |
Shh/Ptc pathway and retinal stem cells in proliferating marginal region of RCS rats during development of retinitis pigmentosa |
| |
| JIAN Qian,XU Hai-wei,XIE Han-ping,YIN Zheng-qin.Shh/Ptc pathway and retinal stem cells in proliferating marginal region of RCS rats during development of retinitis pigmentosa[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(11). |
| |
| Authors: | JIAN Qian XU Hai-wei XIE Han-ping YIN Zheng-qin |
| |
| Institution: | JIAN Qian1,XU Hai-wei2,XIE Han-ping1,YIN Zheng-qin1(1Ophthalmology Research Center,Southwest Hospital,2Department of Physiology,College of Basic Medical Sciences,Third Military Medical University,Chongqing 400038,China) |
| |
| Abstract: | Objective To observe the relationship of activation of retinal cells in the proliferating marginal region of Royal College of Surgeon's rats(RCS rats,an animal model for inherited retinal degeneration),and the expressional changes of sonic hedgehog-patched(Shh/Ptc) signaling during development of retinitis pigmentosa.Methods Totally 28 RCS rats were divided into 4 groups randomly,that is,postnatal day(PND) 15,30,60 and 90 group according to the severity of degeneration(in each group,4 rats for immunofluores... |
| |
| Keywords: | retinitis pigmentosa retinal stem cells cell proliferation Shh/Ptc pathway fluorescent quantitation PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
