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| nm23-H1基因稳定转染肺癌细胞的鉴定 | |
| 引用本文： | 郑海霞,周清华,申东兰,彭安,何艳玲,曹菊秀. nm23-H1基因稳定转染肺癌细胞的鉴定[J]. 癌变．畸变．突变, 2011, 23(2): 145-147. DOI: 10.3969/j.issn.1004-616x.2011.02.015 |
| 作者姓名： | 郑海霞 周清华 申东兰 彭安 何艳玲 曹菊秀 |
| 作者单位： | 1. 北京大学深圳医院体检科,广东,深圳518000 2. 天津医科大学总医院肺外科,天津,300052 3. 北京大学深圳医院肿瘤内科,广东深圳,518000 |
| 基金项目： | 国家自然科学基金资助项 |
| 摘 要： | 目的：通过稳定、高效、低毒的基因转染方法将nm23-H1基因导入该基因缺失的人肺大细胞癌株L9981,从基因及蛋白水平鉴定nm23-H1基因转染肺癌细胞的建立。方法：以JM109为宿主菌,转化质粒载体PCMV-neo-Bam-nm23-H1,制备nm23-H1基因的真核表达载体;利用阳离子脂质体Lipofectamine介导,将nm23-H1基因导入L9981细胞株。采用RT-PCR技术检测转染后nm23-H1基因的表达,并用Western blot技术检测转染后nm23-H1蛋白的表达。结果：转染后的L9981细胞株中可检测到nm23-H1 mRNA及nm23-H1蛋白的阳性表达。结论：建立表达nm23-H1基因的肺癌细胞株L9981,为研究nm23-H1对肺癌恶性转移表型的逆转作用作基础准备。 |
| 关 键 词： | nm23-H1 基因转染 肺癌细胞 肿瘤转移 |
Validation of nm23-H1 gene transfection in lung cancer cells |
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| ZHENG Hai-xia,ZHOU Qin-hua,SHEN Dong-lan,PENG An,HE Yan-lin,CAO Ju-xiu. Validation of nm23-H1 gene transfection in lung cancer cells[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2011, 23(2): 145-147. DOI: 10.3969/j.issn.1004-616x.2011.02.015 | |
| Authors: | ZHENG Hai-xia ZHOU Qin-hua SHEN Dong-lan PENG An HE Yan-lin CAO Ju-xiu |
| Affiliation: | (1.Department of Health Examination,Peking University Shenzhen Hospital,Shenzhen 518000;2.General Hospital of Tianjin Medical University,Tianjin 300052;3.Department of Oncology,Peking University Shenzhen Hospital,Shenzhen 518000,Guangdong,China) |
| Abstract: | OBJECTIVE:nm23-H1 gene was transfected into nm23-H1 deletion human large lung cancer cell L9981 by stable,continuous and high effective gene transfection method.METHODS:nm23-H1 gene expression carrier was established,nm23-H1 was transfected in L9981 cell line and the expression of nm23-H1 was confirmed by gene transduction and protein translation.RESULTS:Transfected cell line showed nm23-H1 gene and protein expressions. CONCLUSION:The expression of nm23-H1 by L9981 cell line provides support for utilizing nm23-H1 as a potential therapy in lung cancer. |
| Keywords: | nm23-H1 |
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