宫颈癌DKK-3基因启动子甲基化检测及其临床意义

目的 检测宫颈癌DKK-3基因启动子甲基化情况并研究其临床意义.方法 采用甲基化特异性PCR检测宫颈癌患者和健康女性宫颈活检组织或术后病理组织DKK-3基因启动子甲基化情况,比较其差异并分析宫颈癌患者DKK-3基因启动子甲基化与临床病理因素的关系.结果 宫颈癌患者DKK-3基因启动子甲基化率显著高于健康女性（P＜0.05）;宫颈癌患者DKK-3基因启动子甲基化率在年龄、吸烟史、酗酒史和病理类型中差异均无统计学意义（P＞0.05）,在HPV感染、分化程度、肿瘤直径、淋巴结转移、远处转移和Figo分期中的差异均有统计学意义（P＜0.05）.结论 宫颈癌患者DKK-3基因启动子甲基化率显著高于健康女性,且与病情及预后密切相关.

Detection and its clinical significance of DKK-3 gene promoter methylation in cervical carcinoma

Objective To detect the condition of DKK-3 gene promoter methylation in cervical carcinoma and study the clinical significance. Methods Methylation specific PCR was used to detect DKK-3 gene promoter＇s methylation in cervical biopsies or postoperative pathological tissue from cervical carcinoma and healthy female.The relationship between clinic pathological factors and DKK-3 gene promoter＇s methylation was analyzed. Results The methylation rate of cervical carcinoma patients was significantly higher than that of healthy female （P〈0.05）.The differences of DKK-3 gene＇s methylation rates in ages,smoking history,alcoholism history and pathological patterns were not statistically significant （P〉0.05）,while the differences of HPV infection,histology differentiation,tumor diameters,lymph node metastasis,metastasis and Figo staging were statistically significant（P〈0.05）. Conclusion The methylation rate in cervi cal carcinoma patients is significantly higher than that of healthy female,which correlates with condition and prognosis.