肾综合征出血热患者血清样本中汉坦病毒RNA检测及其临床意义

目的观察病毒血症在病程各期的变化。方法根据汉坦病毒血清Ⅰ型76-118株及血清Ⅱ型80-39株M基因G1区设计两对公有引物及一条探针，应用RT-nestedPCR方法对57例96份肾综合征出血热(HFRS)患者血清中HV-RNA检测，所有PCR产物用DOT-Blot证实。结果96份样本中68份阳性，阳性率70.8%；在病程第1、2、3周，HV-RNA检测率分别为94.7%、47.5%、25%，P<0.05；重型病人持续2周HV?RNA阳性检测率100%，而轻、中型分别为12.5%及42.8%，P<0.05。结论在HFRS病程中，病毒血症是一个急性过程，与发热期相平行，病毒血症持续时间与病情轻重密切相关。

Detection of Hantan Virus RNA in Hemorrage Fever with Renal Syndrome Patients Serum Samples and Its Clinical Significance

Purpose To observe the viremia duration with the development of the hemorrhage fever with renal syndrome(HFRS) clinical course. Methods According to the published sequence of the M gene G1 segments of Hantan virus (HV) strain 76-118 and Seoul virus strain 80-39,we designed two sets of genus special primer and a probe.We detected the hantavirus RNA by RT nested PCR from 96 HFRS serum samples of 57 patients on the first,second and third week after the onset of illness.All amplified products were confirmed by Dot hybridization. Results Hantavirus RNA was detected in 68 of 96 serum samples,the totle positive rate is 70.8%.The detective rate of HV RNA by the 1st,2nd and 3rd week after the onset of illness was 94.7%,47.5%,25% respectively,( P <0.05).HV RNA was also detected in 4 of 4(100%)severe patients sera on the second week while only 1 of 8(12.5%)mild patients sera and 3 of 7(42.8%)moderate patients sera were detected,( P <0.05). Conclusions HFRS patients have short viremia duration stage,which is parallel to febrile stage.The severity of HFRS may be related to the viremia duration,the more severe clinical presentation,the longer viremia duration.