基因芯片技术检测HBV DNA及拉米夫定耐药株的应用研究

目的 :研究基因芯片技术检测 HBV DNA及拉米夫定耐药株在临床应用中的特异性、实用性。方法 :利用基因芯片法、双脱氧测序法分别对 4 3例服用拉米夫定且 HBV DNA仍为阳性的乙型肝炎 (乙肝 )患者及 10例非乙肝患者的血清进行 HBV DNA和 P区 5 2 8、5 5 2、5 5 5突变位点检测、对比。结果 :两种方法检测 HBV DNA10 0 %相符 ;检测拉米夫定耐药突变株 12 4个位点结果相符 ,5个位点结果不相符 (P >0 .0 5 ) ,提示两种方法检测HBV DNA及 P区 5 2 8、5 5 2、5 5 5突变位点阳性率相等。结论 :利用基因芯片技术检测 HBV DNA及拉米夫定耐药株 ,其特异性可与 DNA测序媲美 ,在混合株检测方面比 DNA测序有更大的优势。其方法简便 ,能大量地对临床标本进行检测 ,可作为临床常规检测手段。

Application Study of Genechip Detecting HBV DNA and Lamivudine Resistant Mutants

Objective:To study the specificity and practicability of genechip detecting HBV DNA and lamivudine resistant mutants in clinic. Methods: The HBV DNA and 528,552,555 mutant sites in P gene of 43 patients treated with lamivudine and HBV DNA positive and the serum of 10 patients with no Hepatitis B were detected and contrasted through genechip method and sequencing method. Results:The HBV DNA detecting results of two methods were identical completely and 124 resistant mutants sites were identical and 5 sites were not( P >0.05),which suggested genechip and sequencing have the same positive ratio in detecting HBV DNA and 528,552,555 mutant sites in P gene. Conclusion: The specificity of genechip method in detecting HBV DNA and lamivudine-resistant mutants was as good as that of sequencing method,and better than sequencing in detecting mix strains. As a simple,convenient,high-flux method,genechip was proposed to become a common detection method in clinic.