| 人乳头瘤病毒16型晚期基因L1的原核表达质粒的构建和鉴定 |
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| 引用本文： | 郭淑元,凌虹,钟照华,庄敏,林道红,曲章义,郭彩玲,谷鸿喜.人乳头瘤病毒16型晚期基因L1的原核表达质粒的构建和鉴定[J].中国地方病学杂志,2000,19(6):472-473. |
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| 作者姓名： | 郭淑元 凌虹 钟照华 庄敏 林道红 曲章义 郭彩玲 谷鸿喜 |
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| 作者单位： | 哈尔滨医科大学,微生物学教研室,黑龙江,哈尔滨,150086 |
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| 基金项目： | 黑龙江省科委重点攻关项目!(G98-CO80 3) |
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| 摘 要： | 目的 构建人类乳头瘤病毒（ＨＰＶ）１６型晚期基因Ｌ１的原核表达质粒，以期从中选出能够高效表达ＨＰＶ１６Ｌ１的重组子，为进一步表达Ｌ１蛋白奠定基础。方法 应用定向克隆策略，将ＨＰＶ１６Ｌ１基因分别克隆到原核表达载体ｐＴｒａｃ９９Ａ和ｐＥＴ－２１ｃ中。结果 通过酶切鉴定选出了重组子ｐＴｒｃ９９Ａ－ＨＰＶ１６Ｌ１和ｐＥＴ－２１ｃ－ＨＰＶ１６Ｌ１。结论 ＨＰＶ１６Ｌ１的原核表达质粒构建成功。
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| 关 键 词： | 人类乳头瘤病毒16 晚期基因L1 基因重组 质粒 |
Construction and identification of late gene L1 of human Papillomavirus type 16 |
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| GUO Shu Yuan,LING Hong,ZHONG Zhao hua,et al.Construction and identification of late gene L1 of human Papillomavirus type 16[J].Chinese Jouranl of Endemiology,2000,19(6):472-473. |
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| Authors: | GUO Shu Yuan LING Hong ZHONG Zhao hua |
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| Abstract: | Objective To construct prokaryotic expression plasmid of Human Papillomavirus type 16(HPV16)L1 gene from which we may select recombinant that can express HPV16 L1 in a high level,and which may make basis for gene expression of L1.Methods The L1 gene of HPV16 Chinese strain is cloned into prokaryotic expression vector pTrc99A and pET 21c by means of determined direction strategy.Results Recombinant plasmid,pTrc99A HPV16L1 and pET 21c HPV16L1,was screened by digestion of restrict endonuclease.Conclusions The construction of prokaryotic expression plasmid of HPV16L1 was achieved. |
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| Keywords: | Human Papillomavirus Type 16(HPV16) Late gene L1 Gene recombinant |
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