针药合用刺激嗅觉系统对血管性痴呆大鼠学习记忆功能及海马自由基系统的影响

[目的]探讨针药合用刺激嗅觉系统对血管性痴呆大鼠学习记忆功能及海马自由基系统的影响作用。[方法]成年SD雄性大鼠48只,体重（300±10）g。随机分为正常对照组、VD模型组、VD嗅球损毁模型组、嗅三针组、丁香酚刺激组和针药合用组,每组8只。制作VD大鼠模型和VD并损毁嗅球大鼠模型,分别通过嗅三针电刺激、丁香酚刺激、针药合用方法,测试大鼠水迷宫学习记忆能力并测定海马MDA含量、GSH-Px和SOD活性。[结果]定位航行试验显示,平均逃避潜伏期和平均游泳路程比较,正常对照组明显短于VD模型组（P〈0.01）;嗅三针组、丁香酚刺激组和针药合用组均明显短于VD模型组（P〈0.01）;针药合用组短于嗅三针组、丁香酚刺激组（P〈0.05）;嗅三针组与丁香酚刺激组相比较,其差异无显著性意义（P〉0.05）;VD模型组与VD嗅球损毁模型组相比较,其差异无显著性意义（P〉0.05）。海马自由基系统检测显示：与正常对照组比较,VD模型组海马MDA含量显著增高（P〈0.01）,而海马GSH-Px和SOD活性明显降低（P〈0.01）;与VD模型组比较,嗅三针组、丁香酚刺激组和针药合用组海马MDA含量显著降低（P〈0.01）,而海马GSH-Px和SOD活性显著增高（P〈0.01）;针药合用组与嗅三针组、丁香酚刺激组比较,海马MDA含量降低（P〈0.05）。[结论]嗅三针和丁香酚均能够显著增强VD大鼠学习记忆功能、能降低海马MDA的含量、并且提高海马GSH-Px和SOD的活。

Effect of Combined Acupuncture and Medicine on Learning-Memory Ability and Free Radical System of Hippocampus in Vascular Dementia Rats

[Objective]To explore the effect of combined acupuncture and medicine on learning-memory abilities, and free radical system of hippoeampus in vascular dementia（VD）rats. [Methods]The 48 Sprague Dawley rats were randomly divided into control （1）, VD model（2）, VD plus removed olfactory bulb model（3）and XIU Three Needles（4）, Eugenol（5）, combined acupuncture attd medicine（6） groups. Morris maze tests were conducted for valuating the learning and memory abilities. The contents of MDA, SOD and GSH-Px activity of hippoeampus were detected. [Results]In comparison with group 1, the average escape latency and travel distance prolonged significantly in group 2. In comparison with group 2, it shortened remarkably in groups 4 and 5. No significant differences were found between groups 1 and 2. In comparison with group 6, it prolonged in groups 4 and 5. No significant differences were found between them. The MDA content of hippoeampus in VD model group was higher than that of control group（P〈0. 05） ;The SOD and GSH-Px activity group 2 were lower than group 1 ;the MDA in group 2 was higher than that of groups 4 and 5 ;The SOD and GSH- Px activity of hippocampus in VD model group were lower than those of groups 4 and 5. No significant differences were found between groups 2 and 3. The MDA in group 6 was lower than that of groups 4 and 5;the SOD and GSH-Px activity in group 6 were higher than groups 4 and 5. No significant differences were found between groups 4 and 5. [Conclusion]The XIU Three Needles and Eugenol increased the learning-memory abilities of VD rats, decreased MDA, and increased SOD and GSH-Px activity of hippocampus.