铁皮石斛的种胚萌发及其离体繁殖研究

目的:研究铁皮石斛种胚萌发和快速繁殖的组织培养技术,为铁皮石斛的繁殖建立最佳培养条件。方法:以成熟的铁皮石斛种胚为研究材料,以1/2MS为基本培养基,接种到含有不同植物生长物质BA(6-苄基腺嘌呤)和NAA(萘乙酸)组合及含有马铃薯提取液、香蕉提取物及活性碳的培养基上,诱导铁皮石斛种胚的萌发、原球茎增殖、分化及试管苗生根。结果与结论:添加20%马铃薯液有利于种胚的萌发,种胚萌发率为79.37%;BA 1.0 mg.L^-1和NAA 0.1 mg.L^-1最有利于原球茎增殖,培养基中添加2%活性碳原球茎增殖倍数高;NAA有助于试管苗的生根,不同含量NAA以0.5 mg.L^-1时试管苗平均根数最多和平均根长最长。

Studies on the seed embryo germination and propagation of Dendrobium candidum in vitro

Objective: To determine optimum culture conditions for the seed embryo culture and rapid propagation of Dendrobium candidum.Method: Seed embryos of D.candidum were incubated in the medium containing a combination of 6-benzylaminopurine(BA) and 1-naphthaleneacetic acid(NAA),potato extract,banana extract and activated carbon in order to induce seed embryo germination,protocorm differentiation,plantlet propagation and plantlet rooting.Result and Conclusion: The maximum embryo germination percentage was obtained in the 1/2 MS media supplemented with 20% potato extract.The 1/2 MS medium supplemented with 1.0 mg·L^-1 BA and 0.1 mg·L^-1 NAA was very beneficial to the protocorm differentiation and propagation of D.candidum.The highest protocorm propagation index was obtained from the medium containing the activated carbon.The highest root numbers and length were observed in plants growing in 1/2 MS medium containing 0.5 mg·L^-1 NAA.