| 含胎牛血清胶原酶消化法培养兔成骨细胞 |
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| 引用本文： | 陈超,李光辉,陈安民. 含胎牛血清胶原酶消化法培养兔成骨细胞[J]. 中国矫形外科杂志, 2004, 12(9): 683-685 |
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| 作者姓名： | 陈超 李光辉 陈安民 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院骨科,武汉汉口解放大道1095#,430030 |
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| 摘 要： | 目的:用含胎牛血清的胶原酶消化胎兔颅盖骨骨组织,进行体外成骨细胞培养.方法:妊娠28d的孕兔,无菌条件下剖腹取出胎兔,然后取胎兔的颅盖骨,剔净、漂洗、剪碎.用含胎牛血清的胶原酶消化,培养成骨细胞.台盼蓝染色测定细胞的存活率,细胞内碱性磷酸酶(ALP)染色鉴定和测定成骨细胞的纯度.结果:用含胎牛血清的胶原酶消化并培养出成骨细胞,台盼蓝染色拒染率高达98%,ALP染色阳性区域不少于95%.结论:用含胎牛血清胶原酶消化胎兔颅盖骨骨组织,培养出的成骨细胞具有典型的成骨细胞特征,且成分单一,存活率高.
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| 关 键 词： | 成骨细胞 细胞培养 含胎牛血清胶原酶 |
| 文章编号： | 1005-8478(2004)09-0683-03 |
| 修稿时间： | 2003-05-28 |
Study on rabbit osteoblasts isolated and digested by collagenase in DMEM containing fetal bovine serum (FBS) |
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| CHEN Chao,LI Guang hui,CHEN An min Orthopedic Department,Tongji Hospital,Tongji Medical College,Huazhong University of science and Technology,Wuhan. Study on rabbit osteoblasts isolated and digested by collagenase in DMEM containing fetal bovine serum (FBS)[J]. The Orthopedic Journal of China, 2004, 12(9): 683-685 |
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| Authors: | CHEN Chao LI Guang hui CHEN An min Orthopedic Department Tongji Hospital Tongji Medical College Huazhong University of science Technology Wuhan |
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| Affiliation: | CHEN Chao,LI Guang hui,CHEN An min Orthopedic Department,Tongji Hospital,Tongji Medical College,Huazhong University of science and Technology,Wuhan 430030 |
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| Abstract: | |
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| Keywords: | Osteoblast Cell culture Collagenase DMEM culture fetal bovine serum |
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