| Ⅰ型脊髓灰质炎病毒型内差异诊断方法的比较 |
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| 引用本文： | 信洪武 张礼壁. Ⅰ型脊髓灰质炎病毒型内差异诊断方法的比较[J]. 中华微生物学和免疫学杂志, 1995, 15(5): 358-362 |
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| 作者姓名： | 信洪武 张礼壁 |
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| 作者单位： | 中国预防医学科学院病毒学研究所，中国预防医学科学院病毒学研究所，浙江省温州市卫生防疫站 100052 北京，100052 北京 |
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| 摘 要： | 将分子生物学方法、免疫学方法及生物学方法相结合，用于Ｉ型脊髓灰质炎病毒型内差异的综合鉴别诊断。分别比较了上述各方法区别野毒株与疫苗相关株，计算符合率的优缺点，为脊髓灰质炎病毒野毒株与疫苗相关株的鉴别诊断提供了依据。
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| 关 键 词： | 脊髓灰质炎病毒 聚合酶链反应 核酸杂交 |
COMPARISON AMONG DIAGNOSTIC METHODS OF INTRATYPIC DIFFERENTIATION OF TYPE I POLIOVIRUSES |
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| Xin Hongwu,Zhang Libi,Ma Wenqi Institute of Virology,Chinese Academy of Preventive Medicine,Beijing. COMPARISON AMONG DIAGNOSTIC METHODS OF INTRATYPIC DIFFERENTIATION OF TYPE I POLIOVIRUSES[J]. Chinese Journal of Microbiology and Immunology, 1995, 15(5): 358-362 |
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| Authors: | Xin Hongwu Zhang Libi Ma Wenqi Institute of Virology Chinese Academy of Preventive Medicine Beijing |
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| Affiliation: | Xin Hongwu,Zhang Libi,Ma Wenqi Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052 |
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| Abstract: | The polymerase chain reaction (PCR),dot-hybridization with digoxigenin-labelled probe,neutralization test with monoclonal antibodies, plaque reduction, neutralization test Rct/40C marker characterization assay and D. marker characterization assay were applied for the intratypic differentiation of type I polioviruses isolated in Wenzhou of China. We compared the accordance rates of intratypic differential on diagnosis by the methods above,and analyzed the advantages and disadvantages of the methods used for the diagnosis. This study is useful for the intratypic differentiation of wild and vaccine-associated strains of polioviruses in China. |
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| Keywords: | Polioviruses Intratypic differecciation Polymerase chain reaction Nucleic acid hybridization Neutralization test |
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