| 抗HCV阳性患者体液中正链和负链HCVRNA的检测及意义 |
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| 引用本文： | 唐振亚 黄元成. 抗HCV阳性患者体液中正链和负链HCVRNA的检测及意义[J]. 中华微生物学和免疫学杂志, 1995, 15(5): 318-320 |
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| 作者姓名： | 唐振亚 黄元成 |
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| 作者单位： | 同济医科大学附属同济医院传染科，同济医科大学附属同济医院传染科，同济医科大学附属同济医院临床免疫研究室，同济医科大学附属同济医院临床免疫研究室，同济医科大学附属同济医院临床免疫研究室 430030 武汉，430030 武汉，430030 武汉，430030 武汉，430030 武汉 |
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| 基金项目： | 国家科委“八五”攻关计划资助 |
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| 摘 要： | 为了解ＨＣＶ感染者血清和体液中ＨＣＶ存在状况，利用ＲＴ－巢式ＰＣＲ方法检测了４２例抗ＨＣＶ阳性患者血清，唾液、精夜或阴道分泌物中的ＨＣＶＲＮＡ。患者血清和体液中ＨＣＶＲＮＡ的检测出率分别为：血清６９．０５％（２９／４２），唾液２３．８１％（１０／４２），精液３６．６％（４／１１），阴道分泌物１５．３８％（２／１３）。
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| 关 键 词： | 正链 HCVRNA 负链 体液 家庭内接触 丙型肝炎 |
DETECTION AND SIGNIFICANCE OF PLUS-AND MINUS HCV RNA IN BODY FLUIDS OF ANTI-HCV POSITIVE PATIENTS |
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| Tang Zhenya,Huang Yuancheng,Wang Youkun,et al Clinical Immunology Research Unit. DETECTION AND SIGNIFICANCE OF PLUS-AND MINUS HCV RNA IN BODY FLUIDS OF ANTI-HCV POSITIVE PATIENTS[J]. Chinese Journal of Microbiology and Immunology, 1995, 15(5): 318-320 |
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| Authors: | Tang Zhenya Huang Yuancheng Wang Youkun et al Clinical Immunology Research Unit |
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| Affiliation: | Tang Zhenya,Huang Yuancheng,Wang Youkun,et al Clinical Immunology Research Unit,Department of Infectious of iseases,Tong Hospital,Tong ji Medical University,Wukan 430030 |
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| Abstract: | The saliva, sperm .vaginal discharge as well as the sera of anti-HCV positive patients were examined for HCV RNA with RT-nested PCR technique. The positive rates of HCV RNA were 23. 81% (10/42) in saliva,36. 36% (4/ 11) in sperm, 15. 38% (2/13) in vaginal discharge and 69. 05% (29/42) in serum .respectively. All body fluids HCV RNA positive patients were serum HCV RNA positive,while no case of body fluids HCV RNA positive was found among those whose sera were HCV RNA negative. This suggests strongly that the status of HCV in body fluids could be closely associated with the existance of HCV in serum. Only 4 patients were minus HCV RNA positive in their saliva,but none in the sperm or vaginal discharge. This meant that HCV probably replicated in saliva glands as reported previously. Our results showed that there were potential risks of transmission of HCV infection through household contact (including sexual contact) with HCV infected patients. The serum HCV RNA positive patients should be subject to antiviral treatment and adequately isolated. |
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| Keywords: | Plus HCV RNA i Minus HCV RNA Household Contact |
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