| 人源性噬菌体抗体库的构建及HBsAg人单抗的筛选 |
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| 引用本文： | 陈竞华,王琰.人源性噬菌体抗体库的构建及HBsAg人单抗的筛选[J].中华微生物学和免疫学杂志,1995,15(3):158-162. |
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| 作者姓名： | 陈竞华 王琰 |
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| 作者单位： | 北京市肿瘤防治研究所，北京市肿瘤防治研究所，解放军海军总医院，北京市肿瘤防治研究所，北京市肿瘤防治研究所，北京市肿瘤防治研究所 100034 北京医科大学临床肿瘤学院，100034 北京医科大学临床肿瘤学院 解放军海军总医院，100034 北京医科大学临床肿瘤学院，100034 北京医科大学临床肿瘤学院，100034北京医科大学临床肿瘤学院 |
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| 基金项目： | 国家科委863计划基金 |
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| 摘 要： | 用聚合酶链伸反应从人外周血淋巴细胞克隆出Ｆｄ段和ｋ链基因，重组到表达载体ｐＣＯＭＢ３中，构建了人源性噬菌体抗体，将乙肝病毒表面抗原固相化对噬菌体抗体库进行了三轮“吸附－洗脱－扩增”的筛选，使特异性噬菌体抗体得到了富集，筛选到了抗ＨＢｓＡｇ的人单抗。
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| 关 键 词： | 噬菌体抗体 HBsAg 单克隆抗体 |
CLONING OF HUMAN McAb AGAINST HBsAg FROM PHAGE ANTIBODY LIBRARY |
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| Chen Jinghua,Wang Yan. Liu Qunying,et al The School of Oncology,Beijing Medical University,Beijing.CLONING OF HUMAN McAb AGAINST HBsAg FROM PHAGE ANTIBODY LIBRARY[J].Chinese Journal of Microbiology and Immunology,1995,15(3):158-162. |
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| Authors: | Chen Jinghua Wang Yan Liu Qunying The School of Oncology Beijing Medical University Beijing |
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| Institution: | Chen Jinghua,Wang Yan. Liu Qunying,et al The School of Oncology,Beijing Medical University,Beijing 100034 |
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| Abstract: | Immunoglobulin heavy chain Fd genes and K chain genes were amplified by RT-PCR from PBLs of a volunteer immunized with HBsAg vaccine. Phage antibody library was constructed with the Fd genes and K chain genes using pCOMB3 as vector. Three rounds selection against coated HBsAg showed specific enrichment of phage antibodies. After the third round selection 19 out of 24 clones exhibited HBsAg binding capacity. The specificity of the clones was verified by ELISAs and competition inhibition ELISA. The success of isolating human anti-HBsAg Fab proved the usefulness of phage display system in human McAb preparation. |
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| Keywords: | Phage antibody library Human McAbj HBsAg |
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