Sarcoplasmic bodies in distal myopathy compared with nemaline rods |
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Authors: | Lars Edström Romuald Wroblewsk |
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Affiliation: | 1. Department of Neurology, Karolinska Hospital, S-104 01 Stockholm Sweden;2. Department of Ultrastructural Research, Wenner-Gren Institute, University of Stockholm, S-113 45 Stockholm Sweden |
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Abstract: | Sarcoplasmic bodies in a late onset distal myopathy and rods in nemaline myopathy have been investigated by electron probe X-ray microanalysis correlated to light microscopy. Sarcoplasmic bodies were glassy, drop-shaped structures up to 10 microns of length and easily distinguished in the scanning mode of electron microscopy performed on thick freeze-dried cryosections. They were found to be source of X-ray spectra characterized by a high sulfur peak. On semithin epoxy sections the sarcoplasmic bodies were observed in the scanning transmission mode as electron-dense structures which, according to X-ray microanalysis, exhibited a high sulphur and osmium content, possibly indicating the presence of sulphydryl groups. The nemaline rods were of 3 different types as judged by their light-microscopical appearance. They were, however, not visible in the scanning mode of electron microscopy performed on freeze-dried unstained cryosections. There were small differences in the elemental composition between different rods within the same biopsy but these were not systematically related to the differences in morphological appearance. |
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Keywords: | Correspondence and reprint requests to: Dr. Lars Edström Department of Neurology Karolinska Hospital S-104 01 Stockholm Sweden. |
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