hMSH2蛋白和P53蛋白表达在胃癌发病中的意义

目的]探讨DNA错配修复基因hMSH2蛋白、抑癌基因P53蛋白表达在胃癌发病中的意义。方法]采用免疫组织化学技术（S—P法）检测63例胃癌组织及32例胃炎组织hMSH2、P53的表达。结果]胃癌组hMSH2蛋白阳性表达率（79．37％）明显高于胃炎组（53．13％）（P〈0．05）；胃癌组P53蛋白阳性表达率（88．89％）明显高于胃炎组（21．88％）（P〈0．05）。hMSH2蛋白在高中分化腺癌、低分化腺癌及黏液癌的阳性表达率分别为73．68％、82．76％和80．00％，P53蛋白阳性表达率分别为100．00％、89．66％和73．33％，两者阳性表达率与胃癌分化程度均无显著性差异（P〉0．05）。hMSH2蛋白和P53蛋白表达呈正相关。结论]DNA错配修复基因hMSH2蛋白及抑癌基因P53蛋白的异常表达可能参与胃癌发生机制。

Significance of gene product expressions of hMSH2 and P53 in gastric carcinogenesis

Objective] To study the relationship of expressions of gene product hMSH2,P53 to gastric carcinogenesis.Method] Immunohistochemical technique was used to detect hMSH2 and P53 protein expressions in 63 cases of gastric carcinomas and 32 cases of gastritis.Results] The positive rate of hMSH2 in gastric carcinoma being 79.37%(50/63) was significantly higher than that in gastritis being 53.13%(17/32)(P<0.05);the positive rate of P53 in gastric carcinoma being 88.89%(56/63) was significantly higher than that in gastritis being 21.88%(7/32)(P<0.05).The positive rates of hMSH2 in well medial differentiation,poor differentiation and mucoid carcinoma were 73.68%,82.76% and 80.00% respectively(P>0.05),and the positive rates of P53 were 100.00%,89.66% and 73.33% respectively(P>0.05).The expression of hMSH2 was positively correlated to the expression of P53 in gastric mucosa including carcinoma and gastritis(r=0.223,P<0.05).Conclusion] The abnormal expressions of mismatch repair gene hMSH2 and tumor suppressor gene P53 may play some roles in the gastric carcinogenesis.