芍芪多苷对小鼠急性化学性肝损伤的保护作用

目的：探讨芍芪多苷（SQDG）对CCl₄致小鼠急性化学性肝损伤模型的保护作用及可能机制,并对SQDG与白芍总苷、黄芪总皂苷单独用药的特点进行比较。方法：建立CCl₄致化学性小鼠急性肝损伤模型,分光光度法检测血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）和肝匀浆中丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）含量,HE染色法对肝脏作病理检查。结果： SQDG能显著降低小鼠血清ALT,AST,MDA水平,升高SOD和GSH-Px酶活性,减轻肝细胞损伤。SQDG中剂量与白芍总苷、黄芪总皂苷单独用药比较,具有较好的肝脏保护作用和抗氧化作用。结论：SQDG对CCl₄致小鼠急性化学性肝损伤模型具有保护作用。

Effects and mechanisms of Shaoqiduogan on mice with chemical liver injury

Objective: To study the therapeutic effects and mechanisms of SQDG on carbon tetrachloride-induced chemical liver injury in mice as well as its possible mechanisms.At the same time the pharmacodynamics of SQDG was compared with TGP or ASTs of effective dose.Method: The model of carbon tetrachloride-induced chemical liver injury in mice was prepared.The levels of ALT,AST,MDA content,SOD and GSH-Px activities in liver homogenate were assayed by spectrophotometry;Meanwhile,hepatic pathological examination was observed.Result: Protective effect of SQDG on carbon tetrachloride-induced chemical liver injury:SQDG was able to significantly decrease serum transaminase levels of chemical liver injury's mice induced by carbon tetrachloride,decreased MDA content and improved the reduced SOD and GSH-px levels in liver homogenate.Furthermore,SQDG also attenuate the area and extent of necrosis and reduce the infiltration of inflammatory cell.Compared with TGP or ASTs of effective dose,SQDG has a better effect on carbon tetrachloride-induced chemical liver injury in mice.Conclusion: SQDG can protect mice injured by carbon tetrachloride-induced chemical.