胆盐对枯否细胞功能的影响

为了证实梗阻性黄疸时高浓度的胆盐对肝枯否 (Kupffer)细胞功能的抑制作用 ,在分离培养的大鼠高纯度的枯否细胞中加入不同终浓度的胆盐 (GCDC) ,并分别进行MTT(噻唑蓝 )、中性红吞噬及TNF(肿瘤坏死因子 )释放的检测。结果表明 :当GCDC <2 5μmol/L时 ,对枯否细胞的活性及功能无明显影响 ;而当GCDC >50 μmol/L时 ,对枯否细胞的活性、吞噬功能及分泌功能均显示出浓度依赖性的抑制作用。提示 ,胆盐对枯否细胞功能具有抑制作用 ,可能在梗阻性黄疸的发展过程中起重要作用

Effect of Bile Salt on Function of Kupffer Cells

To prove that increased bile salts cause the hepatic resticuloendothelial cell dysfunction, collagenase perfusion of the liver and isopycnic sedimentation in a two step Percoll gradient were used to isolate Kupffer cells of wistar rats. After a period of culturing, glycochenodeoxycholate(GCDC) of different terminal concentrations was added respectively. Measuring of MTT, neural red phagocytosis and concentrations of TNF in supernatants was examined to determine how GCDC affect the activity, phagocytic function and TNF release of Kupffer cells. When the concentration of GCDC was less than 25 μmol/L, GCDC had little effect on cultured Kupffer cell. But if GCDC concentration was beyond 50 μmol/L ,functions of Kupffer cells were inhibited significantly by GCDC in a concentration dependent manner. Therefore, the inhibiting function of the bile salts to Kupffer cells probably plays an important role in development of obstructive jaundice.