促凝剂对乙型肝炎病毒实时荧光定量检测影响的评估

目的评估促凝剂对乙型肝炎病毒实时荧光定量检测的影响。方法随机采集2011年10月至2012年3月到西安市中心医院门诊的41例患者每人2管血样,分别为无添加剂普通管和促凝剂管,检测分离血清时间及效果。采用实时荧光定量聚合酶链反应检测乙型肝炎病毒DNA,结果对数值采用SPSS11.5统计软件进行处理,并对促凝剂管标本进行精密度和抗干扰性能评价。结果促凝剂管分离血清平均时间为(4.73±0.86)min,普通管为(14.46±2.27)min,两组数据经配对t检验,差异有统计学意义(P<0.01);促凝剂管乙型肝炎病毒DNA结果对数平均值为(3.86±2.99),普通管组对数平均值为(3.85±2.96),两组经配对t检验,差异无统计学意义(P>0.05),促凝剂管具有高精密度和抗干扰性能。结论促凝剂对乙型肝炎病毒实时荧光定量检测结果准确性没有影响,可减少报告周转时间,值得临床推广和应用。

Assessment of coagulant on hepatitis B virus by real-time fluorescent quantitative detection

Objective To evaluate effect of the coagulant on hepatitis B virus by real-time fluorescent quantitative detection.Methods From October 2011 to March 2012 were randomly collected in Xi＇an city central hospital of 41 patients with each of 2 tubes of blood samples.which were without additives common tube and the coagulant tube.To detected the serum separation time and effect,using real-time fluorescence quantitative PCR for detection of hepatitis B virus DNA,logarithmic mean was performed by SPSS11.5 statistical analysis,and the coagulant tube specimens for precision and anti-jamming performance evaluation.Results Coagulant group separation of serum average time was（4.73±0.86）min,common tube group was（14.46±2.27）min,data from the two groups were paired t test,the difference was statistically significant（P〈0.01）;Logarithmic mean in coagulant group of hepatitis B virus DNA was（3.85±2.96）,and that was（3.86±2.99） in common tube group,using paired t test,the difference between the two groups was not statistically significant（P〈0.05）.coagulant tube had the advantages of high precision and anti-jamming performance.Conclusion The coagulant on hepatitis B virus by real-time fluorescent quantitative detection accuracy is not affected,can reduce the report turnaround time,is worth the clinical promotion and application.