核糖体蛋白L39-L在肺癌1549细胞耐药机制中的作用

目的探讨核糖体蛋白L39-L在肺癌A549细胞对阿霉素类药物耐药机制中的作用。方法以体外培养的阿霉素耐药和敏感人肺癌A549细胞株,啊z01法提取总RNA,LightCyclerRNA扩增法进行荧光定量RT-PCR,检测阿霉素耐药A549细胞株、敏感细胞株之间的RPL39--L基因表达差异。经对人肺癌A549敏感细胞株体外培养、稳定转染RPL39-L基因后,3H—TdR掺人法进行生存率检测分析核糖体蛋白L39-L转染A549细胞、载体转染A549细胞和空细胞株对阿霉素的耐药性差异。结果荧光定量RT-PCR结果显示,耐药性人肺癌A549细胞株比敏感的A549细胞核糖体蛋白L39-L转录水平高7．3倍。1H1TdR掺入测定生存率显示,与质粒载体转染细胞或空细胞相比,转染核糖体蛋白L39-L的细胞表现出增强的阿霉素耐药性。结论核糖体蛋白L39-L基因可育旨在肺癌A549细胞的耐药机制形成机制中有作用。

Effect of RPL39-L on drug resistance of lung cancer A549 cell to amycin

Object To study the effect of RPL39-Lon drug resisitance of lung cancer A549 cell to amycim. Methods The total RNA of in vitro cultured amycin resistant and sensitive lung cancer A549 cell line was isolated by Trizol, and real time quantitative RT-PCR was used to indicate the transcribe difference, viability assay was used to show the amycin resistance difference in RPL39-L transfected A549 cell line, control vector transfected cellline and null-transfected A549 cell line by 1H-TdR. Results The ribosomal protein L39-L trarscripdon level was 7.3 times higher in drug-resistant human lung cancer A549 cell line than in susceptible A549 cell line by quantitative RT-PCRanalysis. The ribosomal protein L39-L transfected cells showed enhanced drug resistance compared to plasmid vector-transfeeted or null-transfected cells determined by methyl tritiated thymidine CH-TdR） incorporation： Corlclusierl The ribosomal protein L39--L gene may have effects on the drug resistance mechanism of lung cancer A549 cells.