| HPLC法测定喉咽清口服液中齐墩果酸的含量 |
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| 引用本文： | 谢谊,李跃辉,唐纯玉,唐代凤,刘玉琴,曹立云,彭艳梅. HPLC法测定喉咽清口服液中齐墩果酸的含量[J]. 中国医药导报, 2014, 0(20): 110-113 |
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| 作者姓名： | 谢谊 李跃辉 唐纯玉 唐代凤 刘玉琴 曹立云 彭艳梅 |
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| 作者单位： | [1]湖南省中医药研究院杨永华名老中医药专家传承工作室,湖南长沙410013; [2]湖南时代阳光药业股份有限公司,湖南永州425000; [3]湖南省中医药研究院杨永华名老中医药专家传承工作室,湖南长沙 410013 |
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| 基金项目： | 湖南省战略性新兴产业重大科技攻关项目（编号2012GK4094） |
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| 摘 要： | 目的建立HPLC-UV法测定酸水解后喉咽清口服液中的齐墩果酸的含量。方法以盐酸-乙醇溶液为水解溶剂,水浴加热回流1 h,再用石油醚（60～90℃）萃取得齐墩果酸;采用高效液相色谱法,以Ultimate XB-C18（4.6 mm×250 mm,5μm）色谱柱,乙腈-水（90∶10）作为流动相,流速为1.0 mL/min,柱温为30℃,检测波长为210 nm。结果齐墩果酸线性范围为2～12μg,平均回收率99.09%,RSD为1.83%。结论所建立方法操作简便、准确、可靠、重现性好,可用于喉咽清口服液中齐墩果酸的含量测定。
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| 关 键 词： | 喉咽清口服液 齐墩果酸 酸水解 含量测定 高效液相色谱法 |
Determination of oleanolic acid in Houyanqing Oral Liquids by HPLC |
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| Affiliation: | XIE Yi;LI Yuehui;TANG Chunyu;TANG Daifeng;LIU Yuqin;CAO Liyun;PENG Yanmei; (1.YANG Yonghua Famous Veteran Teran Doctors of TCM Summon Work Room, Hu＇ nan TCM Academy, Hu＇ nan Province, Changsha 410013, China; 2.Hu＇nan Time Sun Pharmaceutical Corp., Hu＇nan Province, Yongzhou 425000, China; 3.Hu＇nan University of Traditional Chinese Medicine, Hu＇nan Province, Changsha 410208, China) |
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| Abstract: | Objective To establish a method for the determination of oleanolic acid in Houyanqing Oral Liquids after being acid hydrolysis by HPLC. Methods The sample was hydrolyzed with hydrochloric acid- ethanol for 1 hours and extracted with petroleum ether（60-90℃）; the HPLC method was adopted. The Ultimate XB-C18column（4.6 mm×250 mm,5 μm） was used at the column temperature of 30℃. The mobile phase was composed of acetonitrile-water（90∶10） at a flow rate of 1.0 mL/min, the detected wavelength was set at 210 nm. Results The linear ranges of oleanolic acid was 2-12 μg, the average recovery rate was 99.09% with the RSD was 1.83%. Conclusion The developed method is so convenient and has better precision and repeatability. This method can be used to determine the content of oleanolic acid in Houyanqing Oral Liquids. |
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| Keywords: | Houyanqing Oral Liquids Oleanolic acid Acid hydrolysis Content determination HPLC |
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