乌司他丁对大鼠缺血再灌注心肌细胞的保护机制 |
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引用本文: | 王媛媛,曹建,邓莉,陈勤,欧阳先国.乌司他丁对大鼠缺血再灌注心肌细胞的保护机制[J].中国医药导报,2014(20):13-15. |
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作者姓名: | 王媛媛 曹建 邓莉 陈勤 欧阳先国 |
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作者单位: | [1]南昌市第三医院心内科,江西南昌330009; [2]南昌大学第二附属医院麻醉科,江西南昌330006 |
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基金项目: | 江西省卫生厅科技计划项目(编号20142041) |
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摘 要: | 目的观察乌司他丁对心肌缺血再灌注后大鼠心肌细胞的保护机制。方法采用垫扎球囊法结扎冠状动脉制作心肌缺血再灌注的动物模型,30只大鼠随机分为三组:假手术组(n=10)、缺血/再灌注(I/R)组(n=10)和I/R+乌司他丁组(n=10)。检测三组心肌组织谷胱甘肽(GSH)、丙二醛(MDA)及血清标志物肌酸激酶同工酶(CKMB)含量,采用TTC染色确定心肌梗死及缺血范围,并用Western-blot测定炎症因子白细胞介素-6(IL-6)、IL-8的表达。结果与假手术组比较,I/R组中反映氧化损伤程度的MDA明显升高(4.13±3.27)nmol/L比(11.13±2.13)nmol/L],CK-MB也升高(15.21±6.24)U/L比(2752.21±1276.36)U/L],GSH则明显降低(241.42±3.28)mg/L比(184.26±2.14)mg/L],差异均有高度统计学意义(P〈0.01);与I/R组比较,I/R+乌司他丁组MDA(8.25±4.26)nmol/L]、CK-MB(1873.24±621.43)U/L]含量降低,差异有统计学意义(P〈0.05)。I/R+乌司他丁组梗死面积(12.27±4.26)%]小于I/R组(17.12±3.18)%],差异有统计学意义(P〈0.05)。假手术组IL-6、IL-8水平较低(0.412±0.550)、(0.132±0.071)],缺血再灌注后,I/R组IL-6、IL-8表达升高(1.785±0.720)、(0.926±0.247)],差异有高度统计学意义(P〈0.01);与I/R组比较,I/R+乌司他丁组炎性因子IL-6、IL-8水平下降(1.102±0.780)、(0.672±0.240)],差异有统计学意义(P〈0.05)。结论乌司他丁具有抗心肌缺血再灌注损伤作用,其机制可能是通过下调IL-6和IL-8介导的炎性反应而实现。
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关 键 词: | 心肌缺血再灌注 炎症因子 乌司他丁 |
Protection mechanism of Ulinastain on heart ischemia/reperfusion injury in rats |
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Institution: | WANG Yuanyuan;CAO Jian;DENG Li;CHEN Qin;OUYANG Xianguo; (1.Department of Cardiology, the Third Hospital of Nanchang City, Jiangxi Province, Nanchang 330009, China; 2.Department of Anesthesiology, the Second Affiliated Hospital of Nanchang University, Jiangxi Province, Nanchang 330006, China) |
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Abstract: | Objective To observe the protection mechanism of Ulinastain on heart ischemia/reperfusion injury in rats.Methods Experimental model was established by ligation of coronary artery with PTCA balloon, 30 Wistar rats were randomly divided into three groups: sham operation group(n=10); ischemia/reperfusion(I/R) group(n=10); I/R+Ulinastain group(n=10). The GSH and MDA of cardiac muscle tissues, and the serum biomarkers CK-MB in three groups were detected; ischemic and infarct areas in three groups were measured by TCC dyeing; the expression of IL-6 and IL-8 were analyzed by Western-blot analysis. Results Compared with sham operation group, the level of MDA which reflect the degree of oxidative damage in I/R group increased significantly (4.13±3.27)nmol/L vs(11.13±2.13)nmol/L],and the CK-MB was increased (15.21±6.24) U/L vs(2752.21±1276.36) U/L], the value of GSH was decreased significantly, the differences were statistically significant(P〈0.01); compared with I/R group, MDA of I/R+Ulinastain group(8.25±4.26) nmol/L], CK-MB (1873.24 ±621.43) U/L] decreased, the differences were statistically significant(P〈0.05). Infarction area of I/R+Ulinastain group (12.27±4.26)%] was lower than that of I/R group (17.12±3.18)%], the difference was statistically significant(P〈0.05); the levels of IL-6 and IL-8 in sham operation group (0.412±0.550),(0.132±0.071)] were lower, after ischemia reperfusion, the levels of IL-6 and IL-8 in I/R group (1.785±0.720),(0.926±0.247)] was increased, the differences were statistically significant(P〈0.01); compared with I/R group, the level of IL-6 and IL-8 in I/R+Ulinastain group (1.102±0.780),(0.672±0.240)] was decreased, the differences were statistically significant(P〈0.05). Conclusion Ulinastain may protect myocardium from ischemia reperfusion injury by modulating the expression of IL-6 and IL-8 proteins and inhibiting inflammation. |
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Keywords: | Myocardial reperfusion injury Inflammatory factor Ulinastain |
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