| 小剂量氨茶碱对哮喘小鼠气道平滑肌细胞增殖及转化生长因子-β1表达的影响 |
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| 引用本文： | 刘平莉,吕丽丽,夏春伟,李若然,姚红卫,朱述阳. 小剂量氨茶碱对哮喘小鼠气道平滑肌细胞增殖及转化生长因子-β1表达的影响[J]. 实用医学杂志, 2008, 24(1): 27-29 |
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| 作者姓名： | 刘平莉 吕丽丽 夏春伟 李若然 姚红卫 朱述阳 |
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| 作者单位： | 徐州医学院附属医院呼吸科,221008 |
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| 基金项目： | （志谢：感谢徐州医学院动物中心及病理学教研室给予的帮助.） |
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| 摘 要： | 目的:探讨小剂量氨茶碱干预对哮喘小鼠气道平滑肌细胞(ASMC)增殖及转化生长因子-β1(TGF-β1)表达的影响。方法:建立哮喘小鼠气道重塑模型,实验分为对照组、哮喘组、茶碱组。测定支气管肺泡灌洗液中嗜酸性粒细胞计数及活性TGF-β1表达的变化,行增殖细胞核抗原(PCNA)及TGF-β1免疫组化染色,图像分析测定管腔基底膜周长、气管壁总面积、气道平滑肌面积、ASMC核数及TGF-β1在ASMC的灰度值。结果:(1)哮喘组出现管壁、平滑肌层增厚及ASMC增殖等气道重塑的特征,与TGF-β1的表达呈正相关,嗜酸性粒细胞计数明显升高。(2)茶碱组与哮喘组比较炎症反应减轻,管壁、平滑肌层增厚及ASMC增殖减少,TGF-β1表达减弱;与对照组间比较差异无显著性。结论:反复的变应原吸入可导致气道重塑尤其是ASMC增殖,TGF-β1在ASMC增殖中可能具有重要作用,小剂量氨茶碱干预可能减缓气道重塑尤其是ASMC增殖的发生。
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| 关 键 词： | 哮喘 转化生长因子β 氨茶碱 气道平滑肌细胞 |
| 收稿时间： | 2007-05-10 |
| 修稿时间： | 2007-07-21 |
Effects of low-dose aminophylline on proliferation of airway smooth muscle cells and expression of transforming growth factor-β1 in asthmatic mice |
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| LIU Ping-li,LV Li-li,XIA Chun-wei,LI Ruo-ran,YAO Hong-wei,ZHU Shu-yang. Effects of low-dose aminophylline on proliferation of airway smooth muscle cells and expression of transforming growth factor-β1 in asthmatic mice[J]. The Journal of Practical Medicine, 2008, 24(1): 27-29 |
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| Authors: | LIU Ping-li LV Li-li XIA Chun-wei LI Ruo-ran YAO Hong-wei ZHU Shu-yang |
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| Abstract: | Objective To investigate the effects of low-dose aminophylline on proliferation of airway smooth muscle cells (ASMC) and expression of transforming growth factor-β1(TGF-β1) in asthmatic mice. Methods An asthmatic mouse model of airway remodeling was established and the mice were randomized into control group, asthmatic group, and aminophylline group. Eosinophil counts and expression of active TGF-β1 in bronchoalveolar lavage fluid were determined. Perimeter of basement membrane, total bronchial wall area, m... |
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| Keywords: | Asthma Transforming growth factor beta Aminophylline Airway smooth muscle cells |
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