Specific monoclonal antibodies against normal microtubule-associated protein-2 (MAP2) epitopes present in Alzheimer pathological structures do not recognize paired helical filaments |
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Authors: | J. Six U. Lübke M. Mercken M. Vandermeeren C. Ceuterick A. Van de Voorde J. Boons J. Gheuens |
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Affiliation: | (1) Innogenetics, Ghent, Belgium;(2) Laboratory of Neurobiology, Born-Bunge Foundation, University of Antwerp, UIA, Universiteitsplein 1, B-2610 Wilrijk, Belgium;(3) Laboratori of Neuropathology, Born-Bunge Foundation, University of Antwerp, UIA, Universiteitsplein 1, B-2610 Wilrijk, Belgium;(4) Present address: Janssen Research Foundation, Beerse, Belgium |
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Abstract: | Summary We have developed monoclonal antibodies that detect normal microtubule-associated protein-2 (MAP2) epitopes in routinely fixed, paraffin-embedded tissue. The somatodendritic distribution of MAP2 in bovine and human nervous tissue was confirmed with several of these antibodies. Furthermore, some of these antibodies immunohistochemically labeled certain pathological structures in Alzheimer brain, especially neurites in senile plaques. Electron microscopic observations, however, indicate that these MAP2 epitopes are not located in the Alzheimer paired helical filaments themselves, but in amorphous granular structures coexistent with them. While the pathological nature of these structures is undetermined, they may represent artefactual modifications of normal cytoskeletal components. |
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Keywords: | Microtubule-associated protein-2 Somatodendritic distribution Alzheimer's disease Senile plaque neurites Paired helical filaments |
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