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| 模拟微重力培养大鼠胰岛的形态学观察 | |
| 引用本文： | 宋纯,段秀庆,孙野青,李曦,韩礼欧,许评,宋春芳,金连弘. 模拟微重力培养大鼠胰岛的形态学观察[J]. 中华普通外科杂志, 2004, 19(7): 398-400 |
| 作者姓名： | 宋纯 段秀庆 孙野青 李曦 韩礼欧 许评 宋春芳 金连弘 |
| 作者单位： | 1. 150001,哈尔滨医科大学第一临床医学院卫生部细胞移植重点实验室 2. 150001,哈尔滨医科大学第一临床医学院卫生部普外一科 3. 哈尔滨工业大学生命科学院 4. 中国农业科学院哈尔滨兽医研究所生物技术国家重点实验室 5. 哈尔滨医科大学组胚教研室 |
| 基金项目： | 黑龙江省科委“十五”攻关课题基金资助(GC0 2C15 0 ) |
| 摘 要： | 目的应用电子显微镜观察模拟微重力培养对大鼠胰岛形态的影响。方法将新鲜分离和消化的大鼠胰岛分别进行普通培养和模拟微重力条件培养 ,应用扫描电镜和透射电镜观察各组大鼠胰岛的大体结构及超微结构的变化 ,并与新鲜分离的胰岛对照。结果培养 7d时透射电镜下观察微重力组的胰岛形态类似新鲜组的胰岛 ,胞浆内有发育良好的分泌颗粒和丰富的线粒体 ;扫描电镜显示只有微重力组胰岛与胰岛之间形成了许多小洞。结论模拟微重力培养时 ,胰岛的形态保持良好。 |
| 关 键 词： | 胰岛 细胞培养 显微镜检查 电子 |
| 修稿时间： | 2003-08-10 |
Influence of simulated microgravity on rat islets morphology in culture |
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| SONG Chun ,DUAN Xiu-qing,SUN Ye-qing,LI Xi,HAN Li-ou,XU Ping,SONG Chun-fang,JIN Lian-hong. Key Laboratory of Cell Transplantation,Ministry of Health,The First Affiliated Hospital,Harbin Medical University,Harbin ,China. Influence of simulated microgravity on rat islets morphology in culture[J]. Chinese Journal of General Surgery, 2004, 19(7): 398-400 | |
| Authors: | SONG Chun DUAN Xiu-qing SUN Ye-qing LI Xi HAN Li-ou XU Ping SONG Chun-fang JIN Lian-hong. Key Laboratory of Cell Transplantation Ministry of Health The First Affiliated Hospital Harbin Medical University Harbin China |
| Affiliation: | SONG Chun *,DUAN Xiu-qing,SUN Ye-qing,LI Xi,HAN Li-ou,XU Ping,SONG Chun-fang,JIN Lian-hong. *Key Laboratory of Cell Transplantation,Ministry of Health,The First Affiliated Hospital,Harbin Medical University,Harbin 150001,China |
| Abstract: | Objective To observe the influence of simulated microgravity on rat islet. Methods Isolated islet were assigned to flask-culture or bioreactor-culture. Gross structure and ultrastructure of islet were observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Results Islets ultrastructure on 7th day in bioreactor closely resembled fresh islets,with well-formed secretory granules and abundant mitochondria. SEM showed under microgravity islets communicating each other with cavity-like areas. Conclusions The ultrastructure of islets cultured under microgravity closely resembled fresh islets. |
| Keywords: | Islets Cell culture Microscopy electron |
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