环境生物样品中氚分析测量方法研究

目的 建立一种环境生物样品中氚的分析测量方法，并对方法的适用性进行验证，为环境生物中氚分析方法标准的制定提供技术支持。方法 真空冷冻分离生物鲜样制得组织自由水氚试样和生物干样；生物干样与氧气反应后制得有机结合氚试样。用低本底液体闪烁计数器测量氚活度浓度。结果 该方法对组织自由水氚和有机结合氚测试结果的相对标准偏差分别为8.50%和16.3%；组织自由水氚加标测试的加标回收率为96.7%，已知活度浓度试样有机结合氚测试结果的相对误差为-2.38%；组织自由水氚和有机结合氚的探测下限分别为1.07 Bq/kg·鲜和0.0539Bq/kg·鲜。结论 该方法可用于环境生物样品中氚的分析测量。

Study on tritium analysis and measurement method in environmental organisms samples

Objective To establish an analysis and measurement method for tritium in environmental organisms samples and to verify the applicability of the method by experiments, to provide technical support for the formulation of analytical criteria for tritium in environmental organisms. Methods Tissue free water tritium samples and dry organisms samples were prepared by vacuum freezing separation of fresh organisms samples, organically bound tritium samples were prepared by reaction of dry organisms samples with oxygen,and tritium radioactivity was measured with a low background liquid scintillation counter. Results The relative standard deviations of tissue free water tritium and organically bound tritium were 8.50% and 16.3% respectively. The recovery rate of tissue free water tritium via addition of reference materials was 96.7%, and the relative error of organically bound tritium in a given radioactivity sample was -2.38%. The low limit of detection of tissue free water tritium and organically bound tritium were 1.07 Bq/kg·fresh and 0.0539 Bq/kg·fresh respectively. Conclusion This method can be used for the analysis and measurement of tritium in environmental organisms samples.