| 放线菌素D诱导人离体HL7702肝细胞株凋亡及其作用机理 |
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| 引用本文： | 李文君,蔡绍晖,李校堃.放线菌素D诱导人离体HL7702肝细胞株凋亡及其作用机理[J].山东医药,2006,46(14):11-13. |
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| 作者姓名： | 李文君 蔡绍晖 李校堃 |
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| 作者单位： | 1. 暨南大学国家教育部基因组药物工程研究中心,广州,510630
2. 暨南大学国家教育部基因组药物工程研究中心,广州,510630;温州医学院生物与天然药物研究院 |
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| 摘 要： | 目的 探讨放线菌素D（ActD）诱导正常人HL7702肝细胞凋亡及PI3K／Akt信号通路对此过程的作用。方法 采用HL7702正常人肝细胞株，MTT法检测ActD对其存活力的影响；Hoechst33342形态学染色；流式细胞仪检测细胞凋亡情况；Western blot方法检测细胞总Akt（丝氨酸／苏氨酸蛋白激酶）及p-Akt蛋白表达。结果 ActD可诱导HL7702肝细胞凋亡，其浓度在0．25～8μg／ml范围内呈现剂量效应关系；PI3K／Akt特异性抑制剂wortmannin能够增强ActD诱导的肝细胞凋亡。结论 ActD可诱导肝细胞凋亡，其机理可能是抑制PI3K／Akt信号通路。
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| 关 键 词： | 放线菌素D 细胞凋亡 肝细胞 |
| 文章编号： | 1002-266X（2006）14-0011-03 |
| 收稿时间： | 2006-01-09 |
| 修稿时间： | 2006-01-09 |
Actinomycin D induced apoptosis in HL7702 hepatocytes and its mechanism |
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| LI Wen-jun,CAI Shao-hui,LI Xiao-kun.Actinomycin D induced apoptosis in HL7702 hepatocytes and its mechanism[J].Shandong Medical Journal,2006,46(14):11-13. |
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| Authors: | LI Wen-jun CAI Shao-hui LI Xiao-kun |
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| Abstract: | Objective: To investigate the effect of PI3K/Akt signaling on actinomycin D(ActD) induced apoptosis in hepatocytes.Methods: The cytotoxic effects of ActD on HL7702 hepatocytes were measured by the microculture tetrozolium(MTT) assay.Cell apoptosis was evaluated by fluorescence microscopy and flow cytometry.Akt and phospho-Akt were detected by Western blot analysis.Results: ActD caused apoptosis in hepatocytes in a dose-dependent manner,as determined by MTT assays.Phosphatidylinositol-3 kinase(PI-3K) specific inhibitor wortmannin potentiated the apoptotic effect of ActD.Induction of apoptosis correlated with restrain of the PI3K/Akt pathway.Conclusion: ActD can induce apoptosis in hepatocytes,the mechanism may be restrain of the PI3K/Akt pathway. |
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| Keywords: | actinomycin D apoptosis |
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