小剂量紫杉醇对乏氧Hela细胞HIF-1α抑制作用的研究

目的：探讨小剂量紫杉醇对乏氧Hela细胞HIF-1α的抑制作用。方法：细胞乏氧模型采用四甲基偶氮唑蓝（ MTT）快速比色法检测细胞增殖率。细胞免疫荧光法和Western blot检测Hela细胞HIF-1α的表达。结果：紫杉醇使Hela细胞的增殖率降低，但乏氧状态下的Hela细胞增殖率明显高于正常状态下的细胞，两者相比差异有统计学意义（ P〈0.05）。不同浓度紫杉醇可使乏氧状态下的Hela细胞绿色荧光减弱，且呈浓度依赖性。结论：紫杉醇对乏氧和常氧状态下Hela细胞均有抑制作用，乏氧使Hela细胞对紫杉醇有一定的抵抗作用。紫杉醇对乏氧状态下的Hela细胞HIF-1α的表达有抑制作用，呈浓度依赖性。

The study of low dose of paclitaxel repress hypoxia-inducible factor-1α expression in Hela cells under hypoxic conditions

Objective：To investigate whether paclitaxel can inhabit HIF-1α expression in Hela cells under hy-poxia conditions. Methods：The hypoxia model of Hela,MTT assay was used to analyze cells proliferation rate,immu-nofluorescence and Western blot was used to observe the expression of HIF-1α in Hela cells. Results：Paclitaxel make the proliferation ratio of Hela cells down,in the state of hypoxia,the proliferation ratio of Hela cells was signifi-cantly higher than normoxia,there was statistically significant difference between two groups（P〈0. 05）. Different concentration of paclitaxel can make the green fluorescence reduced,and it has dose-dependent effect. Conclusion：Paclitaxel can inhabit the proliferation of Hela cells under normoxia and hypoxia conditions. Hypoxia Hela cells have a certain resistance to paclitaxel. Paclitaxel inhibit HIF-1αexpression in hypoxia Hela cells,and it has dose-depend-ent effect.