猪釉基质蛋白对MC3T3-E1成骨细胞增殖分化的影响

目的：探索釉基质蛋白（ＥＭＰｓ）促进成骨细胞增殖分化的作用，为进一步研究提供基本数据。方法：选择ＭＣ３Ｔ３－ＥＩ成骨细胞株，利用体外细胞培养方法，分别加入不同浓度的ＥＭＰｓ α－ＭＥＭ培养液，于培养０、１、２、３、４、５、６ｄ进行细胞计数，采用ＳＡＳ软件对细胞计数数据作单因素方差分析。结果：ＥＭＰｓ各组细胞计数均高于阴性对照（Ｃ）组（Ｐ〈０．０１）。第２天，１００μｇ／ｍｌ ＥＭＰｓ组的细胞计数高

Influences of Porcine Enamel Matrix Proteins on MC3T3-E1 Osteoblast Proliferation and Diffentiation

OBJECTIVE: Some research suggested that enamel matrix proteins(EMPs) not only induced formation of cementum, but also could promote regeneration of periodontal tissue and alveolar bone. In order to recognize the mechanisms of periodontal regeneration induced by EMPs and provide a basic data for further study, influences of EMPs on osteoblast proliferation and differentiation are explored in this study. METHODS: Porcine enamel matrix proteins were extracted by using acetic acid. MC3T3-E1 osteoblasts were selected and cell culture was used in vitro. EMPs with different concentrations of 50 micrograms/ml, 100 micrograms/ml and 150 micrograms/ml were added separately in alpha-MEM culture medium and compared with negative group and positive group (TGF-beta 1). The count of cell was recorded after 0, 1, 2, 3, 4, 5, 6 days, then the data were analysed statistically by using SAS. RESULTS: The cell count of all EMPs groups were higher than that in the negative control group (P < 0.01), 100 micrograms/ml as well as 150 micrograms/ml EMPs groups were higher than the positive group(TGF-beta 1) on the second day (P < 0.01). The cell count of 100 micrograms/ml EMPs group was not only higher than C and T groups, but also higher than that of other EMPs dose groups on the fifth day. CONCLUSION: The proliferation and differentiation of osteoblasts can be promoted distinctly by enamel matrix proteins.