腺病毒介导的p27kip1基因转移对食管癌细胞周期的影响

用腺病毒为栽体，研究p27kip1基因转移对食管癌细胞周期的影响。方法将携带p27kip1基因的重组腺病毒转染食管癌细胞株Eea9706，用四甲基偶氮唑蓝(MTT)法测定细胞生长抑制效应，免疫细胞化学法检测p27kip1表达，流式细胞仪(FcM)检测细胞周期。结果p27klp1基因转染食管癌细胞株Eca9706后，p27kip1表达增强，细胞生长显著受抑制，G0／G1期细胞比例增加，S期、G2／M期比例降低。结论腺病毒介导的p27kip1基因转移能显著抑制食管癌细胞的生长，使细胞产生G1期阻滞。

Effect of Adenovirus-mediated p27kip1 Gene Transfer on Cell Cycle of Esophageal Carcinoma Cells

Objective To investigate effect of p27kip1 gene transfer mediated by adenovirus vector on the cell cycle of esophageal carcinoma cells. Methods The recombination adenoviral vector carrying p27kip1 gene (Ad-p27kip) was transfected to esophageal carcinoma Eca9706 cells. The effect of p21kip1 gene transfer on the cell growth suppression was measured by MTT method. Expression of p27kip1 was detected by immunocytochemical technique. Cell cycle was analysed by flow cytometry(FCM). Results After Eca9706 cells were transfected with Ad-p27kip1, p27kip1 expression increased, the cell growth was significantly inhibited and the ratio of G 0/G 1 phase cells increased and the cell number of G 2/M and S phases decreased. Conclusion p27kip1 gene transfer mediated by adenovirus can significantly inhibit the growth of esophageal carcinoma cells and cause G 1 phase blocking.