| HPLC法测定白术中白术内酯Ⅱ的含量 |
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| 引用本文： | 李伟,文红梅,崔小兵,张发成.HPLC法测定白术中白术内酯Ⅱ的含量[J].现代中药研究与实践,2005,19(5):35-36. |
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| 作者姓名： | 李伟 文红梅 崔小兵 张发成 |
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| 作者单位： | 南京中医药大学,江苏,南京210029 |
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| 基金项目： | 国家中医药管理局青年基金项目（97Y014） |
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| 摘 要： | 目的研究白术内酯Ⅱ的高效液相色谱测定方法。方法采用反相高效液相色谱法,对白术中白术内酯Ⅱ进行含量测定,色谱柱:Kromasil-C18柱(4.6×250 mm,5μm);流动相:甲醇-水(80∶20);流速:1.0 mL/min;紫外检测波长:276 nm;柱温:30℃;进样量:10μL。结果测定了22种不同白术样品中白术内酯Ⅱ的含量,平均加样回收率为99.8%,RSD为1.2%。结论本法简单、灵敏、结果可靠,可作为白术质量控制的定量方法。
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| 关 键 词： | 白术 白术内酯Ⅱ HPLC |
| 文章编号： | 1004-2199（2005）05-0035-02 |
| 收稿时间： | 2005-08-19 |
| 修稿时间： | 2005年8月19日 |
Determination of AtractylenolideⅡin Atractylodes macrocephala by HPLC |
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| LI Wei,WEN Hong-mei,CUI Xiao-bing,ZHANG Fa-cheng.Determination of AtractylenolideⅡin Atractylodes macrocephala by HPLC[J].Research and Practice on Chinese Medicines,2005,19(5):35-36. |
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| Authors: | LI Wei WEN Hong-mei CUI Xiao-bing ZHANG Fa-cheng |
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| Abstract: | Objective To develop a method to determine the content of atractylenolideⅡ in Atractylodes macrocephala by HPLC.Methods Reverse-Phase HPLC was used to determine the content of AtractylenolideⅡ in Atractylodes macrocephala.The separation was performed on Kromasil-C_(18) column with methanol-water(80∶20) as mobile phase.The flow rate was 1.0 mL·min~(-1) and the wavelength of UV detector was 276 nm.Results 22 Samples were determined.The average recoveries of atractylenolideⅡ were 99.8% with RSD of 1.2%.Conclusion The method is simple,sensitive and reliable.It can be used as quantitative method in quality controlling of Atractylodes macrocephala. |
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| Keywords: | Atractylodes macrocephala atractylenolide HPLC |
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