MMP-14、VEGF、E-cadherin与子宫颈癌生物学行为的相关性研究

目的 探讨正常子宫颈组织和子宫颈癌组织中MMP-14、VEGF和E-cadherin蛋白表达,并研究其与子宫颈癌生物学行为及临床特征的关系.方法 应用免疫组化SP法检测10例正常子宫颈和40例子宫颈癌中MMP-14、VEGF、E-cadherin的表达.结果 与正常子宫颈组织相比,子宫颈癌组织中MMP-14、VEGF蛋白的表达增高,而E-cadherin蛋白的表达降低,差异均有极显著性意义(均P＜0.01);伴盆腔淋巴结转移组MMP-14、VEGF的表达较无淋巴结转移组明显增高,而伴盆腔淋巴结转移组E-cadherin的表达较无淋巴结转移组明显降低(均P＜0.01),VEGF在深肌层浸润组的表达高于浅肌层浸润组,而E-cadherin的表达在深肌层浸润组低于浅肌层浸润组(均P＜0.05);MMP-14、VEGF、E-cadherin异常表达与子宫颈癌的病理学类型及患者年龄无关(均P＞0.05).结论 子宫颈癌组织存在MMP-14、VEGF、E-cadherin的异常表达;MMP-14、VEGF、E-cadherin的表达与子宫颈癌的淋巴结转移和肌层浸润密切相关;检测其在癌组织中的表达水平有助于预测子宫颈癌的浸润和转移.

Relationship between Expression of MMP-14, VEGF, E-cadherin and the Biological Behaviors of Cervical Cancer

Objective To investigate the relationship between the expression of MMP-14,VEGF,E-cadherin and the biological behaviors of cervical cancer.Methods Immunohistochemical SP method was used to detect the expression of MMP-14,VEGF and E-cadherin in 10 cases of normal cervical tissues and 40 cases of cervical cancers.Results The expression rates of both MMP-14 and VEGF in cervical cancer tissues were increased as compared with those in normal cervical tissues(all P<(0.01)).The expression rate of E-cadherin in cervical cancer tissues was lower than that in normal cervical tissues(P<0.01).The expression of MMP-14 and VEGF in cervical cancer tissues with lymph node metastasis was increased markedly as compared with that in cervical cancer tissues without lymph node metastasis,while the expression of E-cadherin was decreased in cervical cancer tissues with lymph node metastasis.The expression of VEGF had a positive relationship with muscular invasion(P<0.05).E-cadherin expression in cervical cancer tissues with invasion of deep muscular layers was lower than that in cervical cancer tissues with invasion of superficial muscular layers(P<0.05).The expression of MMP-14,VEGF and E-cadherin in cervical cancer tissues had no relationship with pathological types and patients' age(P>0.05).Conclusion Abnormal expression of MMP-14,VEGF and E-cadherin occurs frequently in cervical cancer tissues.The expression of MMP-14,VEGF and E-cadherin is closely associated with metastasis and invasion.Detection of these proteins expression may help predict the invasion and metastasis of cervical cancer.