| 高效液相色谱法测定头孢他啶血药浓度 |
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| 引用本文： | 夏东亚,郭涛,陈岩,秦海旭. 高效液相色谱法测定头孢他啶血药浓度[J]. 解放军药学学报, 2007, 23(1): 41-43 |
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| 作者姓名： | 夏东亚 郭涛 陈岩 秦海旭 |
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| 作者单位： | 沈阳军区总医院,药剂科,辽宁,沈阳,110016;沈阳军区总医院,药剂科,辽宁,沈阳,110016;沈阳军区总医院,药剂科,辽宁,沈阳,110016;沈阳军区总医院,药剂科,辽宁,沈阳,110016 |
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| 基金项目： | 全军医学科学技术研究计划科技攻关课题资助项目 |
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| 摘 要： | 目的建立测定头孢他啶血药浓度的方法。方法高效液相色谱法采用Kromasil C18色谱柱（250mm×4．6mm，5μm），以甲醇-磷酸二氢钾溶液（15：85）为流动相，流速1．0ml／min，检测波长254nm。结果头孢他啶吼浆浓度在0．5～200．0mg·L^-1范围内线性笑系良好（r=0．9999）。低、中、高3个浓度的方法回收率为94．91％～105．95％，RSD〈12％；提取回收率为89．50％～92．94％，RSD〈5％。结论本方法简便、准确、精密度好，可用于临床药动学研究。
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| 关 键 词： | 头孢他啶 高效液相色谱法 血药浓度 |
| 文章编号： | 1008-9926（2007）01-0041-03 |
| 收稿时间： | 2006-04-14 |
| 修稿时间： | 2006-05-31 |
Determination of Ceftazidime in Plasma by HPLC |
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| XIA Dong-Ya,GUO Tao,CHEN Yan,QIN Hai-Xu. Determination of Ceftazidime in Plasma by HPLC[J]. Pharmaceutical Journal of Chinese People's Liberation Army, 2007, 23(1): 41-43 |
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| Authors: | XIA Dong-Ya GUO Tao CHEN Yan QIN Hai-Xu |
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| Affiliation: | XIA Dong-Ya, GUO Tao, CHEN Yan, QIN Hai-Xu (1.Department of Pharmacy, General Hospital of Shenyang Military Region, Shenyang 110016 ,Liaoning China) |
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| Abstract: | Aim To establish a method for the determination of ceftazidime concentration in plasma. Method The HPLC separation was performed on Kromasil C18 column （250mm × 4.6mm, 5μm）. The mobile phase was methanol-potassium dihydrogen phosphate （ 15：85 ） at a rate of 1.0mlYmin. The detection wavelength was 254nm. Results The linear range was 0.5 - 200.0mg·L^-1 （ r = 0. 999 9 ）. The method recovery of ceftazidime was 94. 91% - 105.95% （ RSDs lower than 12% ） and the extraction recovery of ceftazidime was 89.50% - 92.94% （ RSDs lower than 5% ）. Conclusion The method appeared to be simple, accurate and precise. It is suitable for clinical pharmacokinetic study. |
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| Keywords: | Ceftazidime HPLC Plasma drug concentration |
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