芹菜素对U251胶质瘤细胞生长及p21、p53蛋白表达的影响

目的 探讨芹菜素对U251胶质瘤细胞生长及p21、p53蛋白表达的影响.方法 常规培养人胶质瘤U251细胞,分别用0、20、40、80 μmol/L芹菜素作用于U251细胞；四甲基偶氮唑盐微量酶反应比色法(MTT法)检测U251细胞增殖抑制率；Western blot检测p53、p21蛋白表达水平.结果 40及80 μmol/L芹菜素抑制人胶质瘤细胞U251的增殖,抑制作用呈剂量和时间依赖性；随着芹菜素浓度的增加,U251中p21和p53表达呈增加趋势.结论 芹菜素可能通过上调p21和p53表达,抑制胶质瘤细胞体外生长.

Effect of apigenin on proliferation and p21, p53 protein expression in glioma cell line U251

Objective To investigate effect ofapigenin on proliferation and p21, p53 protein expression in glioma cell line U251. Methods U251 cells were treated with apigenin at various concentrations （20, 40, 80 Imaol/L）. Cell proliferation was measured by MTT assay on 24, 48 and 72 h. The expression level of p53 and p21 were examined by Western blot. Results Apigenin inhibited the proliferation of U251 cells in a dose-dependent and time-dependent manner. Inhibitory effect was observed at the concentration of 40 and 80 gmol/L. The results of Western blot showed that apigenin up-regulated the expression level of p53 and p21 in a dose-dependent manner. Conclusion The results demonstrate that apigenin has potent inhibitory action on glioma cell proliferation through up-regulating p53 and p21 expression level.