| 加味左金丸中盐酸小檗碱的提取与含量测定 |
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| 引用本文： | 耿铮,车慧.加味左金丸中盐酸小檗碱的提取与含量测定[J].中国医药指南,2013,0(14):401-402. |
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| 作者姓名： | 耿铮 车慧 |
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| 作者单位： | 耿铮 (总后卫生部药品仪器检验所,北京,100071)； 车慧 (总后卫生部药品仪器检验所,北京,100071)； |
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| 摘 要： | 目的建立加味左金丸中盐酸小檗碱的提取和测定方法。方法以50%甲醇-盐酸(100∶1)为溶剂,超声提取30min作为盐酸小檗碱的提取方法。色谱柱CAPCELL PAK C18;流动相:乙腈-0.05mol/L磷酸二氢钠溶液(含0.2%的三乙胺,用磷酸调pH值为3.0)(25∶75);检测波长263nm。结果盐酸小檗碱在进样量0.06672~1.3344mg范围内线性良好r=1,平均回收率为99.27%,RSD=0.71%,(n=6)。结论该方法操作简便,准确可靠,可用于提取和测定加味左金丸中盐酸小檗碱的含量。
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| 关 键 词： | 加味左金丸 盐酸小檗碱 HPLC |
Extraction and Etermination of Berberine Hydrochloride in Jiaweizuojin Pills |
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| GENG Zheng,CHE Hui.Extraction and Etermination of Berberine Hydrochloride in Jiaweizuojin Pills[J].Guide of China Medicine,2013,0(14):401-402. |
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| Authors: | GENG Zheng CHE Hui |
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| Institution: | (Institute for Drug and Instrument Control of Health Dept GLD of PLA, Beijing 100071, China) |
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| Abstract: | Objective To develop a method for the extraction and determination of berberine hydrochloride in Jiaweizuojin Pills. Methods Berberine hydrochloride was extracted with 50% methanol-hydrochloric acid（100 ： 1） in ultrasonic cleaner for 30 minutes. The CAPCELL PAK C18 column was used. The mobile phase was acetonitrile-0.05mol/L sodium dihydrogen phosphate （including 0.2% triethylamine and pH value was adjusted to 3.0）（25 ： 75）. The detection wavelength was at 263 nm. Results The linearity of berberine ydrochloride was fine in the range of 0.06672-1.3344 mg （r=1）. The average recovery was 99.27% and RSD was 0.71% （n=6）. Conclusion The method was convenient and reliable. It was suitable for extraction and determination of berberine hydrochloride in Jiaweizuojin Pills. |
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| Keywords: | Jiaweizuojin pills Berberine hydrochloride HPLC |
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