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正交试验法优选鸡枞多糖的提取工艺
引用本文:冯宁,张元礼,吴世木,陈光明,黄春燕,刘玉丽,娄毕春.正交试验法优选鸡枞多糖的提取工艺[J].检验医学与临床,2009,6(4):257-258.
作者姓名:冯宁  张元礼  吴世木  陈光明  黄春燕  刘玉丽  娄毕春
作者单位:1. 贵州省兴义市人民医院,562400
2. 贵州省黔西南州人民医院
摘    要:目的优选鸡枞多糖的最佳提取工艺。方法采用正交试验方法,以鸡枞多糖得率为考察指标,对影响鸡枞多糖的提取的浸提比、浸提时间、乙醇沉淀浓度等因素进行试验。结果鸡枞多糖的最佳提取条件为2h的浸提时间、1:20的浸提比、85%的乙醇沉淀浓度。结论优选出的提取工艺科学可行。

关 键 词:鸡枞菌  多糖  正交试验

Optimum selection processing study of collybia albuminosa polysaccharopeptide by orthogonal experiment
FENG Ning,ZHANG Yuang-li,WU Shi-mu,CHEN Guang-ming,HUANG Chun-yan,LIU Yu-li,LOU Bi-chun.Optimum selection processing study of collybia albuminosa polysaccharopeptide by orthogonal experiment[J].Laboratory Medicine and Clinic,2009,6(4):257-258.
Authors:FENG Ning  ZHANG Yuang-li  WU Shi-mu  CHEN Guang-ming  HUANG Chun-yan  LIU Yu-li  LOU Bi-chun
Institution:FENG Ning1,ZHANG Yuang-li2,WU Shi-mu1,CHEN Guang-ming1,HUANG Chun-yan2,LIU Yu-li2,LOU Bi-chun2.1.The People's Hospital of Xingyi City,Xingyi,China,2.The People's Hospital of Southwest Prefecture of Guizhou Province,Xingyi 562400
Abstract:Objective To optimize the technology for processing collybia albuminosa polysaccharopeptide. Methods The orthogonal test was employed to test the effects of three factors including lixiviation ratio, lixiviation time and concentration of alcohol on yield of collybia albuminosa polysaccharopeptide. Results The optimal extraction condition was as follows: lixiviation time, 2 hours; lixiviation ratio, 1 : 20 ; and alcohol concentration, 85 %. Conclusion The optimal extraction technology is scientific and feasible.
Keywords:collybia albuminosa  polysaccharides  orthogonal experiment  
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