Resistance of platelet proteins to effects of ionizing radiation |
| |
Authors: | K N Prodouz J W Habraken G Moroff |
| |
Institution: | Food and Drug Administration, Center for Biologics Evaluation and Research, Bethesda, MD 20892. |
| |
Abstract: | Gamma irradiation of blood components prevents lymphocyte-induced graft-versus-host disease after transfusion in immunocompromised individuals. In this report we demonstrate the resistance of blood platelet proteins to gamma radiation-induced protein cleavage and aggregate formation when platelet concentrates were treated with a dose of 5000 rad. Results of one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total platelet protein and cytoskeletal protein preparations indicate that platelet proteins are neither cleaved nor cross-linked under these conditions of irradiation. These results support those of a previous study that documented the lack of any adverse effect of 5000 rad gamma radiation on in vitro platelet properties. |
| |
Keywords: | |
|
|