粉防己碱对溃疡性结肠炎大鼠治疗作用的观察

目的观察粉防己碱对实验性大鼠溃疡性结肠炎的治疗作用。方法采用免疫诱导加化学刺激复合法制作大鼠溃疡性结肠炎模型。将大鼠随机分为正常对照组（C组）、模型组（UC组）和粉防己碱治疗组（TET组）。酶联免疫法检测血清TNF-α和IL-6的含量,肉眼观察肠黏膜的病变并按损伤程度积分,光镜下评价黏膜损伤指数。结果TET组组织损伤积分为3.3±0.6,明显少于UC组（P〈0.01）;光镜下,TET组病理损伤指数为9.7±0.5,明显少于UC组（P〈0.01）;TET组血清样本中TNF-α和IL-6的含量分别为1 086.53±112.55 ng·L^-1和292.91±26.76 ng·L^-1,均明显低于UC组（P〈0.01）。结论粉防己碱对溃疡性结肠炎大鼠模型有较好的治疗作用,其作用机制可能与其降低血清TNF-α和IL-6含量有关。

Therapeutic Effect of Tetrandrine on Ulcerative Colitis Rats

Objective To observe the therapeutic effect of tetrandrine on experimental ulcerative colitis rats. Methods Ulcerative colitis rat model was induced with the compound method of immune induction in combination with chemical stimulation. Rats were normal control group, UC model group and TET groups. randomly divided into three groups as follows： ELISA was used to measure the level of TNF-α and IL-6. Then the rats were killed during the observation of the colonic pathologic changes with naked eyes and for the estimation of the damage scores of colon mucosa. Finally, the colon tissue was fixed to make pathological section and the damage index of colon mucosa was observed under microscope. Results The tissue damage scores of TET group were （0.2 ± 0. 4） significantly lower than that of model control group （3.3±0.6 ,P 〈0.01 ）. Observed under microscope, the pathological damage index of TET group was （2.4±0.5 ） significantly lower than that of model control group （9.7±0.5, P 〈 0. 01 ）. The serum level of TNF-α and IL-6 in TEL group were 1086.53±112.55 and 292.91 ± 26.76 respectively, and were significantly lower than that in the model control group （P 〈 0.01 ）. Conclusion Tetrandrine has protective effect against rat ulcerative colitis, and its mechanism may be related with the reduction of TNF-α and IL-6.