| 大鼠N-乙酰基转移酶Ⅱ(NAT2)活性测定方法的建立 |
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| 引用本文： | 朱俊博,李向阳,袁明,李永平.大鼠N-乙酰基转移酶Ⅱ(NAT2)活性测定方法的建立[J].青海医学院学报,2011,32(2):102-105. |
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| 作者姓名： | 朱俊博 李向阳 袁明 李永平 |
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| 作者单位： | 青海大学医学院 |
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| 摘 要： | 目的 建立大鼠乙酰基转移酶Ⅱ(NAT2)的活性测定方法.方法 借助反相高效液相法,通过对实验条件进行相关设定,建立大鼠尿中咖啡因的3种代谢产物5-乙酰氨基-6-甲酰氨基-3-甲基嘧啶(AFMU)、1-甲基尿酸(1U)和1-甲基黄嘌呤(1X)]测定方法.通过所建方法测定3种代谢产物含量,以AFMU/(AFMU+1U+1...
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| 关 键 词： | N-乙酰基转移酶 反相高效液相色谱 大鼠 |
ESTABLISHMENT OF A METHOD FOR DETERMINING ACTIVITIES OFN-ACETYLTRANSFERASE(NAT2) IN RATS |
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| Zhu Junbo,Li Xiangyang,Yuan Ming,Li Yongping.ESTABLISHMENT OF A METHOD FOR DETERMINING ACTIVITIES OFN-ACETYLTRANSFERASE(NAT2) IN RATS[J].Journal of Qinghai Medical College,2011,32(2):102-105. |
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| Authors: | Zhu Junbo Li Xiangyang Yuan Ming Li Yongping |
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| Institution: | Zhu Junbo,Li Xiangyang,Yuan Ming,Li Yongping(Qinghai University Medical College) |
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| Abstract: | Objective To establish a HPLC method for determining the activities of N-acetyltransferase(NAT2) in Rats.Methods Optimization of experimental condition to determine three metabolites,such as 5-acetylamino-6-formylamino-3-methyluracil(AFMU),1-methyluric acid(1U) and 1-methylxanthine(1X) of] caffeine in rats urine by RP-HPLC method.To infer the activities of N-acetyltransferase(NAT2)by calculating the rate of AFMU/(AFMU+1U+1X).Results The order of accuracy(R>80.6%),precision(R>80.6%)and stability(RSD<6%)of t... |
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| Keywords: | N-acetyltransferase RP-HPLC Rats |
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