大疱性类天疱疮疾病严重程度与BP180抗体水平关系的研究

目的 研究大疱性类天疱疮患者临床疾病严重程度和BP180抗体滴度之间的关系.方法 用BP180NC16a-ELISA分别测定大疱性类天疱疮患者治疗前,病情缓解、糖皮质激素开始减量时和糖皮质激素减量至相当于泼尼松0.5 mg·kg^-1·d^-1时,体内BP180抗体滴度,观察其与患者病情严重程度的一致性.结果 在治疗前,19例大疱性类天疱疮患者的BP180 ELISA平均A值为0.520(0.832～0.372);在病情得到控制、糖皮质激素准备减量时,其BP180 ELISA平均A值为0.405(O.723～0.204);在病情缓解、糖皮质激素减至相当于泼尼松0.5 mg·kg^-1·d^-1时,其BP180 ELISA平均A值为0.215(0.412～0.093).结论 BP180抗体的滴度与大疱性类天疱疮患者疾病严重程度相关,是一种评估疾病病情的有用手段,也可对治疗的有效性作出评价.

Correlation of disease severity with serum titer of anti-BP180 autoantibody in patients with bullous pemphigoid

Objective To investigate the correlation of disease severity with serum titer of anti-BP180 autoantibody in patients with bullous pemphigoid(BP).Methods Twenty patients were enrolled into the study.Blood samples were collected 3-4 times at different stages of treatment process from every patient.Serum titer of anti-BP180 autoantibody was measured with BP180NC16a-ELISA.Correlation analysis of ELISA indices with disease severity was performed.Results Out of the 20 BP patients,19 were positive for anti-BP180 antibody.Mean ELISA OD value of the 19 patients was 0.520(range:0.832-0.372) before treatment,0.405(range:0.723-0.204) when disease was under control and glucocorticosteroid dosage was ready to be reduced,and 0.215(range:0.412-0.093) when glucocorticosteroid dosage was successfully tapered to 0.5 mg·kg^-1·d^-1 of prednisone equivalent.Compared with ELISA indices before treatment,they were significantly reduced after successful therapy(P<0.001).Conclusions Serum titer of anti-BP180 autoantibody is well correlated with disease severity in BP patients.So,it may be used to evaluate the disease severity of and therapeutic effect on BP.