银杏叶提取物对大鼠急性肾缺血再灌注损伤保护作用的实验研究

目的:研究银杏叶提取物对大鼠急性肾缺血再灌注损伤的保护作用。方法:肾缺血45 min再灌注4 h建立大鼠急性肾缺血再灌注损伤模型,用银杏叶提取物预处理,测定肾皮质丙二醛含量、超氧化物歧化酶活性、Na⁺-K⁺-ATP酶和Ca²⁺-ATP酶的活性,血浆肌酐和尿素氮的水平,并观察肾脏病理学改变。结果:缺血再灌注损伤后,肾皮质MDA含量升高,SOD,Na⁺-K⁺-ATP酶和Ca²⁺-ATP酶的活性下降;血浆BUN和Cr水平升高;肾组织出现明显的病理改变。银杏叶提取物预处理能降低肾皮质丙二醛含量;提高超氧化物歧化酶、Na⁺-K⁺-ATP酶和Ca²⁺-ATP酶的活性;降低血浆肌酐和尿素氮水平;肾组织病理改变明显减轻。结论:银杏叶提取物对大鼠急性肾缺血再灌注损伤具有保护作用。

Experimental study about the protective effect of Ginkgo biloba extract on renal acute ischemia-reperfusion injury in rats

Objective:To study the protective effect of Ginkgo biloba extract(EGb) on the kidney in the case of ischemia-reperfusion injury.Method: The model of renal ischemia-reperfusion injury in male rats was made by ligation of left renal artery for 45 min and 4 h of reperfusion.The rats with pretreament were fed with EGb prior to operation.The change of MDA,SOD,Na⁺-K⁺-ATPase,Ca²⁺-ATPase in kidney and BUN,Cr in plasma were determined.The renal pathologic changes were observed.Result: After ischemia-reperfusion,the content of MDA in renal cortex and the levels of BUN,Cr in plasma were increased,the activity of SOD,Na⁺-K⁺-ATPase,Ca²⁺-ATPase in renal cortex were decreased,and the phathological changes induced by ischemia-reperfusion in renal tissues were observed clearly.The pretreatment of rats with EGb coued significantly prevente the reduction of SOD,Na⁺-K⁺-ATPase,Ca²⁺-ATPase activity in renal cortex and the increase of MDA content in renal cortex.,decrease the concenration of BUN,Cr in plasma.the pathological changes of proximal tubular cells in rats kidneys induced by ischemia-reperfusion were also prevented by the pretreatment with EGb.Conclusion:EGb can protect rats from renal injuries caused by ischemia-reperfusion.