姜黄素对博莱霉素致肺纤维化大鼠肺功能改变的影响

目的：观察姜黄素对博莱霉素诱导的肺纤维化大鼠的肺功能的影响。方法：雄性大鼠144只，分为假手术组，模型组，姜黄素高，中，低剂量组(200，100，50 mg·kg^-1·d^-1)，醋酸泼尼松组(0.56 mg·kg^-1·d^-1)，每组24只。除假手术组外其余各组大鼠气管内一次性滴注盐酸博莱霉素，假手术组大鼠气管内一次性滴注等体积的生理盐水。造模后第1天开始灌胃给药，持续到处死动物的前1天。模型组灌服等体积的生理盐水。造模后第7，14，28天时测定肺纤维化大鼠的肺功能及肺组织羟脯氨酸(HYP)。结果：与假手术组相比，7，14，28 d时模型组大鼠吸气阻力(Ri)均明显升高(P<0.01)，最大通气量(MVV)明显降低(P<0.01)，第0.2秒用力呼出容积占用力肺活量百分比(Fev0.2/FVC)进行性升高，用力肺活量(FVC)均明显降低(P<0.01)。最大呼出量(PEF)均明显降低(P<0.01)。模型组大鼠肺组织中HYP的含量均明显升高(P<0.01)。与模型组相比，除Fev 0.2/FVC外，姜黄素高，中，低剂量均可减轻上述变化的趋势，其中以高、中剂量组较为显著(P<0.01，P<0.05)。结论：姜黄素可缓解博莱霉素诱导的肺纤维化大鼠的肺功能改变，并减少肺组织的胶原沉积。

Effects of curcumin on bleomycin-induced damages in pulmonary functions in rats

Objective: To determine the effects of curcumin on bleomycin (BLM)-induced pulmonary fibrosis in rats. Method: One hundred and forty-four male Sprague-Dawley rats were randomized into 6 groups (24 rats in each group, model group, sham group, prednisone group (0.56 mg·kg^-1·d^-1), curcumin with low dose 5 mg group, curcumin with middle dose group 10 mg and curcumin with high dose group 20 mg per 100 g of body weight). Rats in all groups except in sham group were injected with BLM intratracheally. Curcumin with different doses were given by gavage one time everyday for 7, 14 and 28 days. Prednisone were given to rats in prednisone group, po, serving as the positive treatment group. On the 7^th, 14^th, 28^{th day, the lung functions (inspiratory resistance, maximal volutary ventilation, forced vital capacity, Fev 0.2/FVC, peak expiratory flow) were determinated in experimental rats, respectively, and the concentrations of hydroxyproline in lung homogenates of each rat were assayed. Result: Administration of curcumin in different doses improved lung functions of BLM-induced fibrotic rats in the all experimental days; and it decreased the concentration of hydroxyproline in lung homogenates compared with those levels in model control group; and it also lessened the hyperplasia of BLM-induced pulmonary fibrosis in rats. Conclusion: Administration of curcumin can suppress BLM induced pulmonary fibrosis indicated by improved respiratory function, as well as companied with low content of hydroxyproline in lung tissue of rats.}