磷酸化P27kip1的表达与非霍奇金淋巴瘤的恶性相关

目的探讨P27kip110位丝氨酸(Ser10)和187位苏氨酸(Thr187)磷酸化形式的蛋白质表达水平与非霍奇金淋巴瘤(NHL)恶性程度的关系。方法采用免疫组织化学方法检测88例NHL及15例良性增生淋巴结中Ser10、Thr187磷酸化P27kip1和P27kip1蛋白的表达,结合临床及病理资料进行统计分析。结果两种磷酸化位点的P27kip1蛋白在良性增生淋巴结(不包括生发中心)中的阳性率明显低于NHL,随着肿瘤侵袭性的增加,两种磷酸化位点的P27kip1蛋白表达水平增高,与增殖指标Ki-67呈正相关。Thr187磷酸化P27kip1蛋白的阳性率与P27kip蛋白表达水平呈正相关,Ser10磷酸化P27kip1蛋白的阳性率与P27kip1蛋白表达水平表现为负相关。结论磷酸化的P27kip1与NHL的恶性程度有关,联合检测磷酸化P27kip1与P27kip1蛋白的表达水平可为NHL的临床诊断和治疗提供参考。

Expression of phosphorylated of P27kip1 positively correlated with malignancy of non-Hodgkin's Lymphomas

Objective To investigate the expression of the phosphorylated P27kip1 and its correlation with Non-Hodgkin's lymphomas.Methods The expressions of the phosphorylated P27kip1 was detected in lymph nodes by immunohistochemical technique.Results The average labeling indexs(LIs) of phosphorylated P27kip1 in NHLs were significantly higher than those in benign lymph nodes(outside of the germinal center).The expression of phosphorylated P27kip1 in aggressive lymphoma was higher than that in indolent group;and the expression of phosphorylated P27kip1 was significantly correlated with expression of Ki-67.The expression of Thr187 phosphorylated P27kip1 was significantly correlated with expression of P27kip1 while the Ser10 phosphorylated P27kip1 negtively correlated with the expression of P27kip1.Conclusion The high expression of phosphorylated P27kip1 plays key roles in the occurrence and development of NHL;phosphorylated P27kip1 LI and P27kip1 LI are potential parameters to determine the malignance of NHL.