| Bmi-1基因RNAi对胃癌细胞SGC7901及AGS作用的初步研究 |
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| 引用本文： | 肖军,邓长生,夏冰,汤小燕.Bmi-1基因RNAi对胃癌细胞SGC7901及AGS作用的初步研究[J].中华肿瘤防治杂志,2009,16(22):1744-1748. |
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| 作者姓名： | 肖军 邓长生 夏冰 汤小燕 |
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| 作者单位： | 武汉大学中南医院消化内科,湖北,武汉,430071 |
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| 摘 要： | 目的:构建Bmi-1基因的RNAi表达载体,观察其对胃癌细胞SGC7901与AGS增殖、侵袭能力的影响.方法:利用慢病毒表达体系pHelper1.0/pHelper2.0/pGCL-GFP,构建Bmi-1基因的RNAi重组质粒vshRNA-Bmi-1.适时定量PCR和蛋白质印迹法分别检测稳定转染vshRNA-Bmi-1后胃癌细胞中Bmi-1 mRNA及蛋白的表达;MTT法及小室侵袭实验检测胃癌细胞增殖和侵袭能力的变化.结果:稳定转染vshRNA-Bmi-1后SGC7901及AGS细胞Bmi-1 mRNA表达均明显下降,抑制率分别为85%及80%.SGC7901细胞中Bmi-1蛋白表达无明显变化,而在AGS细胞中明显下降.Bmi-1干扰后SGC7901细胞的生长及侵袭能力无明显变化,而AGS细胞增殖减慢、侵袭能力减弱.结论:成功构建Bmi-1基因的RNAi表达载体,vshRNA-Bmi-1重组质粒明显下调Bmi-1 mRNA 在胃癌细胞SGC7901和AGS中的表达.Bmi-1基因的RNAi能抑制胃癌细胞AGS的增殖和侵袭能力.
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| 关 键 词： | 胃肿瘤/病理学 小分子干扰 细胞增殖 肿瘤侵润 |
Effects of RNAi of Bmi-1 gene on gastric carcinoma SGC7901 and AGS cells |
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| XIAO Jun,DENG Chang-sheng,XIA Bing,TANG Xiao-yan.Effects of RNAi of Bmi-1 gene on gastric carcinoma SGC7901 and AGS cells[J].Chinese Journal of Cancer Prevention and Treatment,2009,16(22):1744-1748. |
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| Authors: | XIAO Jun DENG Chang-sheng XIA Bing TANG Xiao-yan |
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| Institution: | XIAO Jun,DENG Chang-sheng,XIA Bing,TANG Xiao-yanDepartment of Gastroenterology,Zhongnan Hospital of Wuhan University,Wuhan 430071,P.R.China |
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| Abstract: | OBJECTIVE:To construct the Bmi1 RNAi expression vector and investigate its influence on the proliferation and invasiveness of gastric carcinoma SGC7901 and AGS cells.METHODS: The recombinant vshRNA-Bmi-1 plasmid of Bmi1 RNAi was constructed by the lentiviral expression system,pHelper1.0/pHelper2.0/pGCL-GFP.Bmi-1 mRNA and protein expressions of stably transfected gastric carcinoma cells were identified respectively by real-time PCR and Western blot analyses and the changes of the proliferation and invasivene... |
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| Keywords: | RNA |
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