YC-1诱导的Survivin表达抑制对食管癌胃癌细胞凋亡和增殖影响的研究

目的:观察YC-1对食管癌Eca109和胃癌SGC-7901细胞中Survivin表达的影响,探讨YC-1对食管癌和胃癌细胞生长的抑制作用.方法:Eca109和SGC-7901细胞分为对照组和YC-1实验组,细胞免疫组化和蛋白质印迹法检测YC-1对Survivin表达的影响,流式细胞仪检测YC-1对细胞凋亡和细胞周期的影响,MTT法检测YC-1对细胞增殖的影响.结果:YC-1抑制Eca109和SGC-7901细胞Sur-vivin的表达.5、10、20、30和40μmol/LYC-1对Eca109细胞48 h增殖抑制率分别为(21.95±2.05)%、(30.69±2.59)%、(50.19±2.28)%、(61.17±2.09)%和(67.50±1.99)%;对SGC-7901细胞的抑制率分别为(9.32±0.61)%、(17.86±1.33)%、(36.95±2.01)%、(47.55±1.68)%和(56.01±1.12)%.10、20和30 μmol/L YC-1作用于Eca109细胞,凋亡率分别为(14.1±0.81)%、(22.1±0.70)%和(24.6±1.01)%;作用于SGC-7901细胞,凋亡率为(15.9±0.75)%、(19.2±0.68)%、(25.4±0.95)%,P<0.05.0、20 μmol/L YC-1作用于Eca109和SGC-7901细胞,S期比例为(21.45±0.91)%,(52.19±3.53)%;(24.41±2.12)%和(54.61±4.12)%,P<0.05.结论:YC-1下调Survivin的表达,诱导食管癌Eca109和胃癌SGC-7901细胞凋亡的发生并抑制细胞增殖,有可能对食管癌和胃癌具有潜在的治疗价值.

Effects of Suppression of Survivin by YC-1 on apoptosis and proliferation of Eca109 and SG0-7901 cell lines

OBJECTIVE:To investigate the effects of 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1) on the expression of Survivin in human esophageal squamous cancer line Eca109 and human gastric cancer line SGC-7901 cells.METHODS:The expressions of Survivin in Eca109 and SGC-7901 cells were examined by immunocytochemical staining and Western blot analysis.The effect of YC-1 on apoptosis and cell cycle was detected by flow cytometry assay.The effect of YC-1 on proliferation was detected by MTT assay.RESULTS:YC-1 ...