几丁聚糖对大鼠体重的影响及与体内瘦素和睾酮关系的研究

目的：为探讨几丁聚糖（chitosan)对大鼠体重的影响及其与体内瘦素和睾酮的关系。方法：给5组大鼠分别喂饲基础饲料，高脂饲料和加不同剂量chitosan的高脂饲料共7周，每周称1次体重，于第7周末断头取血，用放射免疫试剂盒检测血浆中瘦素和血清中酮，结果：chitosan具有明显抑制高脂饲料诱导大鼠肥胖和降低血中睾酮的作用，通过体重与瘦素变化规律的分析，推测chitosan可能对瘦素的分泌具有促进作用。结论：chitosan降低血中睾酮浓度，从而促进瘦素分泌，可能是chitosan抑制高脂饲料诱导大鼠脂胖的部分原因。

Study on the Effect ofChitosan on Rat Body Weight, Concentration of Plasma Leptin, Serum Testosterone and TheirRelationships

Objective The aim of this study was to evaluate effect of chitosan on rat body weight,concentration of plasma leptin,serum testosterone and their relationships.Methods Five groups of rats were respectively given access to base diet,high fat diet and high fat diet containing different doses of chitosan for 7th weeks.All rats were weighed once a week.By the end of 7th week,the animals were sacificed and their blood samples were taken,the concentration of plasma leptin and serum testosterone were determined by RIA Kit method.Results Chitosan prevented the increase of body weight induced by a higth fat diet and lowered serum testosterone concentration.Based on analysis on the changes of body weight and plasma leptin level,it is inferred that chitosan could give rise to the augment of leptin in plasma.Conclusion The concentration of serum testosterone reduced by chitosan could stiumlate the secretion of leptin,which could be in part responsible for the inhibiting the increase of body weight induced by high fat diet.