羧甲基壳聚糖及其复合物对根管内粪肠球菌的作用评价

目的：探讨羧甲基壳聚糖(carboxymethyl chitosan，CMCS)及其复合物对根管内粪肠球菌的抑菌作用，为在根管治疗方面的应用积累数据。方法：建立粪肠球菌感染根管模型，随机分为A、B、C、D 4组，分别将2%氯己定、140 mg/mL羧甲基壳聚糖氯己定合剂、5 mg/mL羧甲基壳聚糖、Ca(OH)2糊剂注满相应组的各样本根管腔内，培养7 d。封药前、后取样，分别接种在BHI琼脂平皿上，48h后计数菌落形成单位(CFU/mL)。采用SPSS13.0软件包对数据进行统计学分析。结果：封药前，各组根管内细菌量差异无显著性(P>0.05)。封药后即刻，各组根管内细菌量的减少差异显著(P<0.05)。A组与B组的抗菌活性显著优于C组、D组。结论：2%氯己定与140 mg/mL羧甲基壳聚糖氯己定合剂对粪肠球菌具有较强的抗菌活性。

An in vitro evaluation of the effect of carboxymethyl chitosan and its composites against Enterococcus faecalis in the root canal

PURPOSE: To evaluate the bacteriostasis effect of carboxymethyl chitosan and its composites on intracanal E. faecalis. METHODS: The pattern of E. faecalis infecting root canal was established, and then divided into 4 groups(A, B, C and D).They were filled with 2% chlorhexidine solution, 140 mg/mL mixture of carboxymethyl chitosan and chlorhexidine solution, 5 mg/mL carboxymethyl chitosan solution and calcium hydroxide paste respectively and incubated for 7 days. Samples obtained before and after the intracanal medication were plated onto BHI media to determine the colony-forming units (CFU/mL) after 48 hours. The data were analysed using SPSS 13.0 software package. RESULTS: Before intracanal medication, the variance of bacterial counts were not significantly different (P>0.05). After medication, the four groups showed significant difference in bacterial counts immediately(P<0.05).The antimicrobial effects of A and B group were better than group C and D. CONCLUSIONS: The antibiotic activity of 2% chlorhexidine solution and 140 mg/L mixture of carboxymethyl chitosan and chlorhexidine solution to E.faecalis were better.