| 弓形虫致密颗粒蛋白的免疫反应性评价 |
| |
| 引用本文： | 尹志奎,詹合琴,许兵红,刘世国.弓形虫致密颗粒蛋白的免疫反应性评价[J].新乡医学院学报,2005,22(4):318-320. |
| |
| 作者姓名： | 尹志奎 詹合琴 许兵红 刘世国 |
| |
| 作者单位： | 新乡医学院药理学教研室; 新乡医学院寄生虫学教研室; 新乡医学院寄生虫学教研室 河南新乡; 河南新乡 |
| |
| 摘 要： | 目的对弓形虫的重组蛋白谷胱甘肽巯基转移酶致密颗粒蛋白(GSTGRA7)的免疫反应性进行评价。方法异丙基βD硫代半乳糖苷(IPTG)诱导大肠埃希菌BL21(pGEX4T1/GRA7)表达,表达产物进行十二烷基硫酸钠(SDS)变性蛋白质电泳,分别以人抗弓形虫阳性血清和兔抗弓形虫阳性血清为一抗进行WesternBlotting分析。用GST亲和层析柱纯化重组蛋白,以此蛋白作为包被抗原,酶联免疫吸附试验(ELISA)检测抗弓形虫阴性、阳性血清。结果重组蛋白GSTGRA7能被兔抗弓形虫阳性血清、人抗弓形虫阳性血清所识别。该蛋白能与人抗弓形虫阳性血清、兔抗弓形虫阳性血清特异结合,而与抗弓形虫阴性血清无反应。结论弓形虫重组蛋白GSTGRA7具有良好的免疫反应性。
|
| 关 键 词： | 弓形虫 致密颗粒蛋白7 重组蛋白 免疫反应性 酶联免疫吸附试验 |
| 文章编号： | 1004-7239(2005)04-0318-03 |
Evaluation on the immunoreactivity of the recombinant protein GST-GRA7 of Toxoplasma gondii |
| |
| YIN Zhi-kui,ZHAN He-qin,XU Bing-hong,et al.Evaluation on the immunoreactivity of the recombinant protein GST-GRA7 of Toxoplasma gondii[J].Journal of Xinxiang Medical College,2005,22(4):318-320. |
| |
| Authors: | YIN Zhi-kui ZHAN He-qin XU Bing-hong |
| |
| Institution: | YIN Zhi-kui1,ZHAN He-qin1,XU Bing-hong2,et al |
| |
| Abstract: | Objective To evaluate on the immunoreactivity of the recombinant protein GST-GRA7 of toxoplasma gondii.Methods The Escherichia coli.BL21,which had recombinant plasmid pGEX-4T-1/GRA7, was induced by IPTG. The protein was analyzed by SDS-PAGE and Western blotting with positive sera. .Then the protein was purified and used as a diagnostic antigen for ELISA to test positive sera and negative sera. Results The protein could react with human/rabbit positive sera analyzed by Western blotting. Conclusion The recombinant protein GST-GRA7 has very good immunoreactivity. |
| |
| Keywords: | toxoplasma gondii dense granule protein7 (GRA7) recombinant protein immunoreactivity enzyme-linked immunosorbent assay |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《新乡医学院学报》浏览原始摘要信息 |
| 点击此处可从《新乡医学院学报》下载免费的PDF全文 |
