乙型肝炎病毒核心启动子热点突变与HBcAg存在状态

目的：研究乙型肝炎病毒（ＨＢＶ）核心启动子（ＢＣＰ）区１７６２位和１７６４位热点突变对ＨＢｅＡｇ存在状态的影响。方法：采用错配引物的ＰＣＲ结合限制性内切酶技术检测９０例不同ＨＢｅＧａ状态的ＨＢＶ无症状携带者的ＨＢＶＢＣＰ热点突变。结果：６０例ＨＢｅＡｇ阴性者中ＨＢＶＢＣＰ热点突变者２６例（４３．３％），其中２０例伴有ＨＢＶ前Ｃ区（Ｐｒｅ－Ｃ）区１８９６位热点突变；３０例ＨＢｅＡｇ阳性者中仅３例（１

Relationship between hot spot mutation in hepatitis B virus basic core promotor and HBeAg status]

OBJECTIVE: This paper was to study the influence of hot spot mutation in hepatitis B virus (HBV) basic core promotor (BCP) (nt1762 and nt1764) on HBeAg status of asymptomatic HBV carriers. METHODS: Mismatched PCR was used to amplify the fragment of HBVBCP and the fragments were analysed by restrict enzyme assay. Ninety cases of HBV infection were tested for hot spot mutations in HBVBCP. RESULTS: Twenty-six (43.3%) of 60 asymptomatic HBV carriers with HBeAg negative were found to have hot spot mutations in HBVBCP, among which 20 cases were accompanied by the mutation of nt 1896 in HBV Pre-C region. Hot-spot mutation in HBVBCP, however, occurred only in 3(10%) of 30 asymptomatic HBV carriers with HBeAg positive. For further investigation the HBVBCP hot spot mutations in asymptomatic HBV carriers without mutation of HBV Pre-C region were studied. Six(31.6%) of 19 asymptomatic HBV carriers with HBeAg negative were found to have hot spot mutation in HBVBCP. Two(7.1%) of 28 asymptomatic HBV carriers with HBeAg positive had hot spot mutations in HBVBCP, and there was a statistically significant difference between two groups. CONCLUSIONS: Hot spot mutations in HBVBCP is common in the HBV carriers with HBeAg negative and usually accompanies with nt1896 mutation of HBV Pre-C region. It is possible that Hot spot mutation in HBVBCP is a new reason of HBeAg negative HBV infection.