温灸诱发循经高温线形态学和组织酶化学相关机制研究

目的:研究经络温度现象的结构和功能基础,探讨循经高温线的形成机制,了解经络现象的实质。方法:选用健康成年家兔16只,应用红外热像仪寻找和定位温灸家兔“后三里”穴后诱发出的循胃经高温线,对高温线上和经线外组织进行取材,观察和比较经线内外乙醛酸诱发荧光反应以及乙酰胆碱酯酶、乳酸脱氢酶的含量。结果:温灸家兔“后三里”穴能诱发出循足阳明胃经的高温线。循经高温线上分布的乙醛酸诱发出的荧光物质(肾上腺素能神经递质)比经线外少。循经高温线上乙酰胆碱酯酶活性较强,高温线外酶活性则较弱,两者比较有显著性差异(P<0.01)。循经高温线上乳酸脱氢酶活性比较强,高温线外乳酸脱氢酶活性则较弱,两者比较有显著差异(P<0.01)。结论:①温灸后所诱发出循经高温线现象是一种较可靠的经络温度现象。②肾上腺素能神经递质较少、能量代谢旺盛、乙酰胆碱含量增加引起的血管扩张均是循经高温线的形成机制。

Study on Zymologic and Morphologic Mechanisms of Moxibustion-induced High-temperature Line along the Meridian

Objective: To research the mechanism of moxibustion-induced high-temperature lines along the meridian from both structure and function. Methods: 16 adult Japanese big-ear white rabbits were used in the present study. Following shearing hairs at the lateral side of the lower limb, moxibustion was applied to bilateral “Zusanli”(ST 36) , 5～6 minutes later, thermogram along the “Stomach Meridian” was taken by infrared ray thermal instrument. The part of the lower limb showing higher-temperature band monitored by thermogramer was frozen and removed rapidly, the sample including skin, subcutaneous tissue and muscle was then cut into sections (10 μm or 20 μm) stained with histochemical techniques [glyoxylic acid (SPG)] for displaying acetylcholine esterase (AChE) and lactic dehydrogenase (LDH) activities. Results: After moxibustion of “Zusanli”(ST 36), higher temperature traces appeared alone the running course of the Stomach Meridian. Under light microscope, the bright green fluorescence intensity in the tissue sample of higher-temperature band (meridian course) was weaker, while that of extra-meridian tissue samples stronger. The optic density (OD) values of AChE and LDH in the slices of the higher-temperature tissues (along the Stomach Meridian) and non-higher-temperature tissues (extra-meridian) were 0.617±0.265 vs 0.514±0.169, and 0.265± 0.08 vs 0.199±0.05 (each n=32) respectively, displaying significant differences between meridian tissue and extra-meridian tissue in the OD values (P<0.01). It suggests that under higher-temperature state of the running course of meridian, the level of monoamines (adrenergic transmitter being predominant) was lower, and the activity of AChE and LDH higher in the tissues along the meridian than that in the tissues beyond the meridian (P<0.01). Conclusion: Moxibustion induced high temperature line reaction alone the Stomach Meridian is related to the decrease of monoamines (adrenergic transmitter) and the increase of AChE level and potentiation of energy metabolism.