利用组织微阵列研究RASSF1A和survivin基因在非小细胞肺癌中的表达及其临床意义

目的利用高通量组织微阵列技术并结合免疫组化检测RASSFlA和survivin基因在非小细胞肺癌（NSCLC）组织中的蛋白表达，探讨RASSF1A和survivin基因的表达与NSCLC临床病理特征的关系及其临床意义。方法构建包含NSCLC组织和正常肺组织的组织微阵列，免疫组织化学S—P法检测RASSF1A和stlrvivin基因在NSCLC与正常肺组织中的蛋白表达。结果RASSF1A蛋白在NSCLC组织中的阳性表达率（46．8％，37／79）显著低于正常肺组织（92．9％，13／14）（P〈0．001）。临床I期和临床Ⅱ期NSCLC组织的RASSF1A蛋白阳性表达率显著高于临床Ⅲ期（P〈0．0001，P〈0．0001）。淋巴结转移NSCLC组织中RASSF1A蛋白阳性表达率显著低于无淋巴结转移NSCLC（P〈0．05）。NSCLC组织中survivin蛋白阳性表达率（75．8％，62／79）显著高于正常肺组织（0％，0／14）（P〈0．0001）。临床I期和临床Ⅱ期NSCLC组织的survivin蛋白阳性表达率显著低于临床Ⅲ期（P=0．003，P=0．001）。进一步研究发现NSCLC组织中RASSF1A与survivin蛋白的表达存在显著性负相关（r=-0．780；P〈0．0001）。结论RASSF1A蛋白在NSCLC组织中的表达缺失可能促进NSCLC的发生、发展，其有望成为评估肺癌患者预后的重要参考指标。survivin蛋白参与NSCLC的发生、发展，过表达survivin蛋白的NSCLC肺癌患者可能预后不良。RASSF1A和survivin基因表达的失平衡可能在NSCLC的发生、发展中具有重要作用。

Expression of RASSFlA and survivin genes in the non-small cell lung cancer and the clinical significance by tissue microarray

Objective To investigate the association between the expression of RASSF1A and survivin proteins in non-small cell lung cancer （NSCLC） and their clinicopathological features. Methods NSCLC tissue microarrays （TMA） including NSCLC tissue and normal lung tissues from non-tumor lung samples was constructed. Immunohistoehemistry S - P was used to detect the expressions of RASSF1A and survivin proteins in the NSCLC tissue microarrays containing 79 cases of NSCLC and 14 normal lung tissues. Resuits NSCLC TMA including 93 samples was constructed successfully. The positive expression of RASSF1A in NSCLC （46. 8%, 37/79） was significantly lower than that of the normal lung tissues （92. 9%, 13/14） （P 〈 0. 001）. The percentage of RASSF1A protein expression in the stage I and stage Ⅱ of NSCLC was significantly higher than that in the stage Ⅲ （P 〈0. 0001, respectively）. The percentage of RASSF1A in NSCLC with lymph node metastasis was significantly lower than that in cases without lymph node metastasis （ P 〈 0. 05 ）. The positive expression of survivin in NSCLC （78.5%, 62/79） was significantly higher than that in the normal lung tissues （0%, 0/14） （P 〈 0. 0001 ）. The percentage of survivin protein expression in the stage I and stage Ⅱ of NSCLC was significantly lower than that of in the stage Ⅲ（P = 0. 003, P = 0. 001 ）. Furthermore, there was a significantly negative correlation between expression of RASSF1A protein and that of survivin protein in NSCLC （r = - 0. 780; P 〈 0. 0001 ）. Conclusions The loss expression of RASSF1A protein in NSCLC relates to its lymph node metastasis and TNM stage. The overexpression of survivin in NSCLC is positively related to its TNM stage. The disturbed balance expression between RASSF1A and survivin proteins may play an important role in the development and progression of NSCLC.